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RISS 인기검색어
- 검색키워드
- 저자 : Aliasghar Hamidi (검색결과 2 건)
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Covalent Immobilization of Trypsin on a Novel Aldehyde-Terminated PAMAM Dendrimer
Hamidi, Aliasghar,Rashidi, Mohammad R.,Asgari, Davoud,Aghanejad, Ayuob,Davaran, Soodabeh Korean Chemical Society 2012 Bulletin of the Korean Chemical Society Vol.33 No.7
Dendrimers are a novel class of nonlinear polymers and due to their extensive applications in different fields, called versatile polymers. Polyamidoamine (PAMAM) dendrimers are one of the most important dendrimers that have many applications in nanobiotechnology and industry. Generally aldehyde terminated dendrimers are prepared by activation of amine terminated dendrimers by glutaraldehyde which has two problems, toxicity and possibility of crosslink formation. In this study, novel aldehyde-terminated PAMAM dendrimer was prepared and used for covalent immobilization of trypsin by the aim of finding a special reagent which can prevent crosslinking and deactivation of the enzyme. For this purpose aminoacetaldehydedimethylacetal (AADA) was used as spacer group between aldehyde-terminated PAMAM and trypsin.The findings of this study showed that immobilization of trypsin not only resulted higher optimal temperature, but also increased the thermal stability of the immobilized enzyme in comparison to the free enzyme.
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Covalent Immobilization of Trypsin on a Novel Aldehyde-Terminated PAMAM Dendrimer
Aliasghar Hamidi,Mohammad R. Rashidi,Davoud Asgari,Ayuob Aghanejad,Soodabeh Davaran 대한화학회 2012 Bulletin of the Korean Chemical Society Vol.33 No.7
Dendrimers are a novel class of nonlinear polymers and due to their extensive applications in different fields, called versatile polymers. Polyamidoamine (PAMAM) dendrimers are one of the most important dendrimers that have many applications in nanobiotechnology and industry. Generally aldehyde terminated dendrimers are prepared by activation of amine terminated dendrimers by glutaraldehyde which has two problems, toxicity and possibility of crosslink formation. In this study, novel aldehyde-terminated PAMAM dendrimer was prepared and used for covalent immobilization of trypsin by the aim of finding a special reagent which can prevent crosslinking and deactivation of the enzyme. For this purpose aminoacetaldehydedimethylacetal (AADA) was used as spacer group between aldehyde-terminated PAMAM and trypsin.The findings of this study showed that immobilization of trypsin not only resulted higher optimal temperature, but also increased the thermal stability of the immobilized enzyme in comparison to the free enzyme.
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