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Monir Teymoori,Ali Morsali,Mohammad Reza Bozorgmehr,S. Ali Beyramabadi 대한화학회 2017 Bulletin of the Korean Chemical Society Vol.38 No.8
Using density functional theory, noncovalent interactions and four mechanisms of covalent functionalization of 6-thioguanine anticancer drug onto γ-Fe2O3 nanoparticles have been investigated. Quantum molecular descriptors of noncovalent configurations were studied. It was specified that binding of 6-thioguanine onto γ-Fe2O3 nanoparticles is thermodynamically suitable. Hardness and the gap of energy between LUMO and HOMO of 6-thioguanine are higher than the noncovalent configurations, showing the reactivity of 6-thioguanine increases in the presence of γ-Fe2O3 nanoparticles. 6-thioguanine can bond to γ-Fe2O3 nanoparticles through NH2 (k1 mechanism), NH in six-membered ring (k2 mechanism), NH in five-membered ring (k3 mechanism), and S (k4 mechanism) groups. The activation energies, the activation enthalpies and the activation Gibbs free energies of these reactions were calculated. Thermodynamic data indicate that k3 mechanism is exothermic and spontaneous and can take place at room temperature. These results could be generalized to other similar drugs.
Techniques for Evaluation of LAMP Amplicons and their Applications in Molecular Biology
Esmatabadi, Mohammad javad Dehghan,Bozorgmehr, Ali,zadeh, Hesam Motaleb,Bodaghabadi, Narges,Farhangi, Baharak,Babashah, Sadegh,Sadeghizadeh, Majid Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.17
Loop-mediated isothermal amplification (LAMP) developed by Notomi et al. (2000) has made it possible to amplify DNA with high specificity, efficiency and rapidity under isothermal conditions. The ultimate products of LAMP are stem-loop structures with several inverted repeats of the target sequence and cauliflower-like patterns with multiple loops shaped by annealing between every other inverted repeats of the amplified target in the similar strand. Because the amplification process in LAMP is achieved by using four to six distinct primers, it is expected to amplify the target region with high selectivity. However, evaluation of reaction accuracy or quantitative inspection make it necessary to append other procedures to scrutinize the amplified products. Hitherto, various techniques such as turbidity assessment in the reaction vessel, post-reaction agarose gel electrophoresis, use of intercalating fluorescent dyes, real-time turbidimetry, addition of cationic polymers to the reaction mixture, polyacrylamide gel-based microchambers, lateral flow dipsticks, fluorescence resonance energy transfer (FRET), enzyme-linked immunosorbent assays and nanoparticle-based colorimetric tests have been utilized for this purpose. In this paper, we reviewed the best-known techniques for evaluation of LAMP amplicons and their applications in molecular biology beside their advantages and deficiencies. Regarding the properties of each technique, the development of innovative prompt, cost-effective and precise molecular detection methods for application in the broad field of cancer research may be feasible.