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RISS 인기검색어
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- 저자 : Ajithan Chandrasekaran (검색결과 1 건)
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Vasudevan Venkatachalam,Sathish Dorairaj,Ajithan Chandrasekaran,Sathish Selvam,Manickavasagam Markandan 한국식물생명공학회 2021 Plant biotechnology reports Vol.15 No.4
The production of transgenic watermelon through Agrobacterium tumefaciens-mediated transformation with in vitro regen- eration system is a time-consuming, labor-intensive and genotype-dependent process. To acquire large number of transgenic watermelons in a shorter period of time, the half-seed explants were infected with Agrobacterium strain EHA 105 harboring pCAMBIA1301 with bar and gus genes. The factors affecting in planta transformation efficiency, such as co-cultivation duration, acetosyringone concentration, sonication duration and vacuum infiltration were assessed in the present study. The half-seed explants were sonicated for 3 min and 2 min vacuum infiltrated in Agrobacterium suspension and co-cultivated for 3 days with 100 µM acetosyringone showed maximum transformation efficiency. The transformed watermelon plants were selected against BASTA® and GUS, PCR, Southern hybridization analysis confirmed the transgene integration. The amenability of this established protocol was analyzed on four genotypes, in which the response of all genotypes was posi- tive, whereas Arka manik showed the higher transformation efficiency of 17%. The transformation protocol developed in the present study is efficient, economical and expeditious without the taking part of any tissue culture phases and produce a large number of transgenic lines within a short period of 56 days.
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