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Jelli Venkatesh,Chandrama Prakash Upadhyaya,유재웅,Ajappala Hemavathi,김두환,Reto J. Strasser,박세원 한국원예학회 2012 Horticulture, Environment, and Biotechnology Vol.53 No.4
L-Ascorbate plays a vital role in the alleviation of salinity stress in crop plants. Overexpression of the ascorbate pathway enzyme D-galacturonic acid reductase in transgenic potato plants confers improved tolerance to various abiotic stresses. These transgenic potato plants were further studied for their primary photosynthetic performances under salinity stress. The changes in primary photochemistry of PSII induced by salinity stress were studied using JIP-test. Analysis of the fast phase chlorophyll a fluorescence transients indicated that there was a differential effect of salinity stress on different sites of the photosynthetic machinery. The transgenic potato leaves exhibited a gain in the ability for restraining the energy loss when they were imposed by salinity stress. These observations suggest that under salinity stress, the photosynthetic energy conservation in the transgenic plants was more effective than in the wild-type plants.
Upadhyaya, Chandrama Prakash,Venkatesh, Jelli,Gururani, Mayank Anand,Asnin, Leonid,Sharma, Kavita,Ajappala, Hemavathi,Park, Se Won Kluwer Academic Publishers 2011 Biotechnology letters. Vol.33 No.11
<P>Salt-tolerance was studied in transgenic potato. It was conferred by overexpression of ascorbate pathway enzyme (D-galacturonic acid reductase, GalUR). As genetic engineering of the GalUR gene in potato enhances its ascorbic acid content (L-AsA), and subsequently plants suffered minimal oxidative stress-induced damage, we now report on the comprehensive aptness of this engineering approach for enhanced salt tolerance in transgenic potato (Solanum tuberosum L. cv. Taedong Valley). Potatoes overexpressing GalUR grew and tuberized in continuous presence of 200 mM of NaCl. The transgenic plants maintained a higher reduced to oxidized glutathione (GSH:GSSG) ratio together with enhanced activity of glutathione dependent antioxidative and glyoxalase enzymes under salinity stress. The transgenics resisted an increase in methylglyoxal that increased radically in untransformed control plants under salinity stress. This is the first report of genetic engineering of ascorbate pathway gene in maintaining higher level of GSH homeostasis along with higher glyoxalase activity inhibiting the accumulation in methylglyoxal (a potent cytotoxic compound) under salt stress. These results suggested the engineering of ascorbate pathway enzymes as a major step towards developing salinity tolerant crop plants.</P>