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- 저자 : Aggarwal Shilpi (검색결과 2 건)
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Aggarwal, Shilpi,Karimi, I. A.,Lee, Dong Yup Royal Society of Chemistry 2011 Molecular bioSystems Vol.7 No.11
<P>The remarkable catabolic diversity of <I>Rhodococcus erythropolis</I> makes it an interesting organism for bioremediation and fuel desulfurization. However, a model that can describe and explain the combined influence of various intracellular metabolic activities on its desulfurizing capabilities is missing from the literature. Such a model can greatly aid the development of <I>R. erythropolis</I> as an effective desulfurizing biocatalyst. This work reports the reconstruction of the first genome-scale metabolic model for <I>R. erythropolis</I> using the available genomic, experimental, and biochemical information. We have validated our <I>in silico</I> model by successfully predicting cell growth results and explaining several experimental observations in the literature on biodesulfurization using dibenzothiophene. We report several <I>in silico</I> experiments and flux balance analyses to propose minimal media, determine gene and reaction essentiality, and compare effectiveness of carbon, nitrogen, and sulfur sources. We demonstrate the usefulness of our model by studying a few <I>in silico</I> mutants of <I>R</I>. <I>erythropolis</I> for improved biodesulfurization, and comparing the desulfurization abilities of <I>R. erythropolis</I> with an <I>in silico</I> mutant of <I>E. coli</I>.</P> <P>Graphic Abstract</P><P>This work reports the reconstruction of the first genome-scale metabolic model for <I>R. erythropolis</I> using the available genomic, experimental, and biochemical information. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c1mb05201b'> </P>
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Jangra Sumit,Dhall Heena,Aggarwal Shilpi,Mandal Bikash,Jain Rakesh Kumar,Ghosh Amalendu 한국응용곤충학회 2020 Journal of Asia-Pacific Entomology Vol.23 No.2
Thrips palmi is an important insect pest of vegetables and ornamental crops worldwide. Besides direct damage caused by feeding, it transmits several tospoviruses in a persistent-propagative manner. Eggs of T. palmi are microscopic and embedded within plant tissue by the sharp ovipositor of adult female. In the present study, an artificial oviposition setup has been standardized for T. palmi. Eggs of T. palmi were harvested in sterile water between two thin membranes. The developmental stages of T. palmi embryo were studied starting from oviposition up to hatching by inverted and confocal reflection microscopy. Energids were homogeneously distributed at an early stage of development. The anterior end of the egg curved with a constriction post 38 h. Initiation of tissue organization, mouthparts, appendages, compound eyes were observed at different time points. Appendages were well developed and segmentation was prominent post 70 h. The embryo was completely developed at around 80 h and hatched by 86 h post oviposition at 28 °C temperature. The study first time reports the embryonic development of T. palmi that would be helpful in detailed investigations of thrips developmental biology and evolution.
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