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- 저자 : Afsharifar Alireza (검색결과 2 건)
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Sazegari Sima,Niazi Ali,Shahriari-Ahmadi Farajollah,Afsharifar Alireza 한국원예학회 2022 Horticulture, Environment, and Biotechnology Vol.63 No.5
Terpenoid indole alkaloids (TIA) are important pharmaceuticals produced in low amounts through the terpenoid indole alkaloid pathway in Catharanthus roseus. Production of these alkaloids is controlled by biosynthetic and regulatory genes. CrMYC1 is a chief transcription factor that regulates terpenoid indole alkaloid production. In this study, we cloned CrMYC1 and overexpressed it in C. roseus hairy roots to investigate its effect on the production of the TIAs ajmalicine and catharan- thine. qRT-PCR results revealed that CrMYC1 expression in transgenic CrMYC1-overexpressing hairy root lines was 3–6 times higher than that of the wild-type hairy root control line. Also, we detected a significant increase in strictosidine β-D- glucosidase (SGD) expression, a critical enzyme in TIA biosynthesis, in the overexpression lines. HPLC analysis showed that CrMYC1 overexpression significantly improved the accumulation of ajmalicine (13 to 14-fold) and catharanthine (3 to 4.4-fold) compared to the control hairy root line. These results provide insight into the role of this understudied transcription factor in TIA pathway regulation and emphasize further research objectives.
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Ali Pourkhaloee,Morteza Khosh-Khui,Paul Arens,Hassan Salehi,Hooman Razi,Ali Niazi,Alireza Afsharifar,Jaap van Tuyl 한국원예학회 2018 Horticulture, Environment, and Biotechnology Vol.59 No.6
Tulip ( Tulipa L.) is one of the most important ornamental geophytes in the world. Analysis of molecular variability of tulipsis of great importance in conservation and parental lines selection in breeding programs. Of the 70 genic microsatellites, 15highly polymorphic and reproducible markers were used to assess the genetic diversity, structure, and relationships among280 individuals of 36 wild and cultivated tulip accessions from two countries: Iran and the Netherlands. The mean valuesof gene diversity and polymorphism information content were 0.69 and 0.66, respectively, which indicated the high discriminatorypower of markers. The calculated genetic diversity parameters were found to be the highest in wild T . systolaStapf (Derak region). Bayesian model-based STRU CTU RE analysis detected fi ve gene pools for 36 germplasms whichcorresponded with morphological observations and traditional classifi cations. Based on analysis of molecular variance, toconserve wild genetic resources in some geographical locations, sampling should be performed from distant locations toachieve high diversity. The unweighted pair group method with arithmetic mean dendrogram and principal component analysisplot indicated that among wild tulips, T . systola and T . micheliana Hoog exhibited the closest relationships with cultivatedtulips. Thus, it can be assumed that wild tulips from Iran and perhaps other Middle East countries played a role in the originof T . gesneriana , which is likely a tulip species hybrid of unclear origin. In conclusion, due to the high genetic variabilityof wild tulips, they can be used in tulip breeding programs as a source of useful alleles related to resistance against stresses.
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