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Rapid fabrication of highly porous and biocompatible composite textile tubular scaffold for vascular tissue engineering

Abdal-hay, Abdalla,Memic, Adnan,Hussein, Kamal H.,Oh, Yi Seul,Fouad, Mohamed,Al-Jassir, Fawzi F.,Woo, Heung-Myong,Morsi, Yosry,Mo, Xiumei,Ivanovski, Saš,o Elsevier 2017 European polymer journal Vol.96 No.-
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Abstract Three dimensional (3D) constructs for vascular tissue engineering applications require scaffolds with highly porous architectures, high biocompatibility and mechanical stability. In this paper, composite fibrous tubular scaffolds composed of different ratios of poly(epsilon-caprolactone) (PCL) and polyamide-6 (PA-6) were simultaneously deposited layer by layer by employing the air jet spinning (AJS) textile technique. Specifically, we report on the optimal parameters for the fabrication of composite porous scaffolds that allow for precise control over the general scaffold architecture, as well as the physical and mechanical properties of the scaffolds. In vitro cell culture study was performed to investigate the influence of polymer composition and scaffold architecture on the adhesion of EA.hy926 human endothelial cells onto the fabricated scaffolds. The cell culture results indicated that a composite scaffold with low PA-6 fibrous content is the most promising substrate for EA.hy926 adhesion and proliferation. Based on the present findings, these highly porous composite tubular constructs support endothelial cell migration and cellular infiltration, and hence represent promising nano-fibrous scaffolds for vascular tissue engineering. Highlights <UL> <LI> PCL/Nylon 6 dual) fibrous 3D tissue scaffolds were synthesized for vascular grafts. </LI> <LI> Highly and tunable hybrid porous fibrous tissue scaffold was obtained by AJS. </LI> <LI> EA.hy926 EC was sued to determine the biocompatibility of tissue scaffolds. </LI> <LI> Dual scaffold provided a favorable attachment and proliferation of EA.hy926 human EC. </LI> <LI> Dual scaffold at low N6 content induced highest biocompatibility compared to others. </LI> </UL> Graphical abstract [DISPLAY OMISSION]
2
Carbon rich fly ash and their nanostructures

Numan Salah,Sami S. Habib,Zishan H. Khan,Ahmed Alshahrie,Adnan Memic,Attieh A. Al-ghamdi 한국탄소학회 2016 Carbon Letters Vol.19 No.-
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  ScienceON
  
  스콜라
  
  코리아스칼라
Carbon rich fly ash was recently reported to have compositions that are ideal for use as a precursor and catalyst for carbon nanotube growth. This fly ash powder is mostly composed of pure carbon, predominantly present as sp2. In this work, the effect of sonication time on the morphology and structural properties of carbon rich fly ash particles is reported. The obtained results show that ultrasound treatment is an effective tool for producing ultrafine particles/fragments with higher porosity, which might be suitable for the adsorption of gasses. Moreover, carbon nanoparticles (CNPs) of this fly ash were produced in parallel using the ball milling technique, and were evaluated as reinforcements for epoxy based composites. These CNPs have almost spherical shapes with particle sizes of around 30 nm. They were found to have strong C=O carbonyl group bonds, which might be generated during the ball milling process. The tensile testing results of a fly ash CNP reinforced epoxy composite showed significant improvements in the mechanical properties, mainly in the stiffness of the polymer. The stiffness value was increased by around 23% of that of neat epoxy. These CNPs with chemically active groups might also be useful for other applications.