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      • KCI등재

        Morphological and genetic variation of thirty Iranian Dendranthema (Dendranthema grandiflfl orum) cultivars using multivariate analysis

        Shirin Taghipour,Abdollah Ehtesham Nia,Hamed Khodayari,Hasan Mumivand,Mohammad Reza Shafiei 한국원예학회 2021 Horticulture, Environment, and Biotechnology Vol.62 No.3

        Analysis of genetic diversity in dendranthema is necessary to improve this important ornamental species. In this study, 30Dendranthema grandifl ora cultivars were assessed based on 21 phenotypic traits and 13 ISSR loci based on a completelyrandomized block design with three replications in Iran. Phenotypic traits such as plant height, lateral branch number, leafnumber, leaf area, and fl ower number had high (i.e., > 45%) phenotypic and genotypic coeffi cients of variation, indicatingthe potential of these traits for improving through targeted selection in breeding programs. All of the ISSR markers werepolymorphic, and 86 alleles were identifi ed across the cultivars. The average polymorphic information content was 0.35with a Shannon’s information index of 0.29, indicating high levels of genetic variation in the tested cultivars. The clusteranalysis based on Jaccard similarity indices (J) grouped 30 genotypes into 10 major clusters. The closest relation wasobserved between the ‘Oran’ and ‘Taban3′ cultivars (with 91% similarity), while ‘Fariborz’ and ‘Farahnaz’ cultivars werefar apart from others (with 38% similarity). All cultivars were clearly diff erentiated by their ISSR fi ngerprints. T(GA)9 and(AGC)5GA markers had the highest molecular indices, which could be used for future breeding programs of dendranthema. Eleven cultivars were classifi ed separately into four groups (A–D), and the remaining 19 cultivars were placed in the otherfour groups (E–H). The fi ndings of this study will aid in parental selection studies for hybridization programs of dendranthemain future.

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