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RISS 인기검색어
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- 저자 : Abdellatif Bahaji (검색결과 2 건)
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Abdellatif Bahaji,Alejandro Caldero´n-Urrea,Thihan Padukkavidana,Robert T. Gaeta,Carlos Tristan,Glenda W. Polack 한국식물생명공학회 2012 Plant biotechnology reports Vol.6 No.4
Plant parasitic nematodes are devastating to agricultural production. Recent estimates indicate that losses due to nematode infestation can reach US$ 125billion per year worldwide. Further aggravating the problem is the use of chemicals, such as methyl bromide. Even though methyl bromide is still the best tool currently available to combat nematode infestation, it has been targeted by international treaties for elimination from worldwide agriculture due to its deleterious impact on the environment. Therefore, alternatives are urgently needed to eliminate the threat of both parasitic nematodes and hazardous pesticides. In an attempt to develop novel strategies for nematode control, we produced and characterized transgenic tobacco plants constitutively expressing the ced-4 gene from Caenorhabditis elegans and exposed homozygous lines to the plant parasitic nematode Meloidogyne incognita. Five transgenic lines tested showed a significant reduction in the number of nematode-induced galls formed. These tolerant tobacco lines displayed high levels of ced-4expression (determined via a competitive reverse transcription-PCR assay) and the presence of CED-4 (determined by Western blot analysis with anti-CED-4antibodies). In addition, protein extracts from transgenic ced-4 plants restored CED-3 activity of the ced-4 mutant C. elegans protein extracts to wild-type levels, indicating that the CED-4 protein produced in these transgenic plants is functional. We suggest that programmed cell death genes may provide an alternative to control plant parasitic nematodes.
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Alejandro Caldero´n-Urrea,Fumiko Yamamoto,Thihan Padukkavidana,Abdellatif Bahaji,Davis W. Cheng,Glenda W. Polack 한국식물생명공학회 2012 Plant biotechnology reports Vol.6 No.4
Transgenic tobacco plants expressing the Caenorhabditis elegans programmed cell death gene ced-9, in both sense and antisense orientations, were produced using Agrobacterium tumefaciens-mediated transformation. The generated transgenic tobacco plants were tested for resistance to the root-knot nematode Meloidogyne incognita by measuring gall formation, size of galls generated, and the ability of juvenile-2 (J2) to hatch. Results showed that expression of ced-9 gene in either sense (ced-9F) or antisense (ced-9R) orientation in hemizygous transgenic tobacco plants induced prevention of M. incognita proliferation (as measured by gall number reduction) and J2hatching. Furthermore, the results also showed that ced-9R in homozygous transgenic tobacco plants prevented J2hatching, whereas ced-9F homozygous transgenic tobacco plants lost nematicidal function. Although our study demonstrates that expression of either ced-9R or ced-9F genes in tobacco plants significantly reduces infection by M. incognita, further investigation is required to understand the specific mechanisms involved for this control. It is possible that the nematode resistance seen with both sense (ced-9F) and antisense (ced-9R) sequences is the result of two independent mechanisms, one acting on invading nematodes and the other acting during embryogenesis of M. incognita, ultimately resulting in plant protection.
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