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Mahmodi, Farshid,Kadir, J.B.,Puteh, A.,Pourdad, S.S.,Nasehi, A.,Soleimani, N. The Korean Society of Plant Pathology 2014 Plant Pathology Journal Vol.30 No.1
Genetic diversity and differentiation of 50 Colletotrichum spp. isolates from legume crops studied through multigene loci, RAPD and ISSR analysis. DNA sequence comparisons by six genes (ITS, ACT, Tub2, CHS-1, GAPDH, and HIS3) verified species identity of C. truncatum, C. dematium and C. gloeosporiodes and identity C. capsici as a synonym of C. truncatum. Based on the matrix distance analysis of multigene sequences, the Colletotrichum species showed diverse degrees of intera and interspecific divergence (0.0 to 1.4%) and (15.5-19.9), respectively. A multilocus molecular phylogenetic analysis clustered Colletotrichum spp. isolates into 3 well-defined clades, representing three distinct species; C. truncatum, C. dematium and C. gloeosporioides. The ISSR and RAPD and cluster analysis exhibited a high degree of variability among different isolates and permitted the grouping of isolates of Colletotrichum spp. into three distinct clusters. Distinct populations of Colletotrichum spp. isolates were genetically in accordance with host specificity and inconsistent with geographical origins. The large population of C. truncatum showed greater amounts of genetic diversity than smaller populations of C. dematium and C. gloeosporioides species. Results of ISSR and RAPD markers were congruent, but the effective maker ratio and the number of private alleles were greater in ISSR markers.
Farshid Mahmodi,J. B. Kadir,A. Puteh,A. Nasehi,N. Soleimani,S. S. Pourdad 한국식물병리학회 2014 Plant Pathology Journal Vol.30 No.1
Genetic diversity and differentiation of 50 Colletotrichumspp. isolates from legume crops studied through multigeneloci, RAPD and ISSR analysis. DNA sequence comparisonsby six genes (ITS, ACT, Tub2, CHS-1, GAPDH, and HIS3)verified species identity of C. truncatum, C. dematium andC. gloeosporiodes and identity C. capsici as a synonymof C. truncatum. Based on the matrix distance analysis ofmultigene sequences, the Colletotrichum species showeddiverse degrees of intera and interspecific divergence (0.0 to1.4%) and (15.5-19.9), respectively. A multilocus molecularphylogenetic analysis clustered Colletotrichum spp. isolatesinto 3 well-defined clades, representing three distinctspecies; C. truncatum, C. dematium and C. gloeosporioides. The ISSR and RAPD and cluster analysis exhibited a highdegree of variability among different isolates and permittedthe grouping of isolates of Colletotrichum spp. into threedistinct clusters. Distinct populations of Colletotrichum spp. isolates were genetically in accordance with host specificityand inconsistent with geographical origins. The largepopulation of C. truncatum showed greater amounts ofgenetic diversity than smaller populations of C. dematiumand C. gloeosporioides species. Results of ISSR and RAPDmarkers were congruent, but the effective maker ratio andthe number of private alleles were greater in ISSR markers.
Mohd Ridhwan Adam,Mohd Hafiz Dzarfan Othman,Siti Hamimah Sheikh Abdul Kadir,Muthia Elma,Tonni Agustiono Kurniawan,Ahmad Fauzi Ismail,Mohd Hafiz Puteh,Azeman Mustafa,Mukhlis A. Rahman,Juhana Jaafar,Hud 한국화학공학회 2021 Korean Journal of Chemical Engineering Vol.38 No.4
This work investigates the effectiveness of ammoniacal nitrogen (NH4 +-N) removal from contaminated water by adsorptive hollow fiber ceramic membrane (HFCM) derived from naturally made clinoptilolite. The technological value of this work is the simple mechanism of the adsorptive HFCM in removing gaseous ammonia in water by combining adsorption and separation. To test the technical feasibility of this proposed technology, clinoptilolite HFCM was fabricated via phase inversion-based extrusion/sintering technique and characterized by SEM and water permeation flux. The produced HFCM corresponds to the desired morphology of the asymmetric structure (dense and void formations) with outstanding adsorption performance of NH4 +-N. The effects of the HFCM’s operational parameters on its removal are examined in terms of membrane dosage and isothermal studies. The adsorption isotherm behavior exhibited that the adsorption process fitted the Freundlich isotherm model with outstanding removal performance even at trace concentration of ammonia. The low amount used by HFCM (4.75×104m2) resulted in over 96% ammonia removal, indicating a low cost of adsorption process. The regeneration of saturated HFCM suggests an outstanding recovery of the HFCM for its subsequent use for NH4 +-N removal. This study also reveals the potential of adsorptive HFCM as a simple and cost-effective technology for ammonia removal from wastewater.