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DEAE - Cellulose 에 의한 산양유 Casein 의 분별 및 이화학적 성질에 관한 연구
김병철,김세헌,한경식,임동현,전우민,황광연 한국유가공기술과학회 2000 Journal of Dairy Science and Biotechnology (JMSB) Vol.18 No.1
This experiment was carried out to study fractionation and physicochemical characteristics of caprine casein. Acid caseins obtained from caprine colostral and normal milk were analyzed by chymosin treatment and fractionated by DEAE-cellulose column chromatography with linear gradient and electrophoresis. Protein, fat and lactose of caprine normal milk were 2.70±0.27%, 3.82±0.51%, and 4.10±0.29%, respectively. More non-protein nitrogen(NPN) was released by chymosin treatment from caprine colostral casein than normal casein. The electrophoretic pattern of caprine casein was not similar to that of bovine casein. Caprine normal casein was fractionated by DEAE-cellulose column chromatography with a 0.08∼0.18 M NaCl linear gradient into 5 peaks with the proportion of 5.27%, 26.07%, 25.97%, 30.40% and 12.29%, respectively. In order to identify the pure fraction, the chymosin-treated caprine normal casein was fractionated by DEAF-cellulose column chromatography with a 0.08∼0.18 M NaCl linear gradient into 6 peaks with the proportion of 17.06%, 9.10%, 17.85%, 20.11%, 27.03% and 8.85%, respectively.