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Human Cytomegalovirus 감염시 UL47 promoter 활성의 분석과 Escherichia coli에서 발현되는 UL47 단백질의 분석
현종준,김기호,김홍진 중앙대학교 약학연구소 1997 약학 논총 Vol.11 No.-
In order to study of HCMV(human cytomegalovirus) UL47 promoter activity, we have used the transient transfection assay method. We cloned the -20∼-325bp region to be expected as UL47 promoter part into the indicator plasmid which includes the β-glucuronidase gene. In the transient promoter activation experiment, the promoter activity was increased up to 48-fold in the HCMV(AD169 strain) infected cells. This result indicates that the viral infection is necessary for the UL47 promoter activation occurred in only late times. In order to express UL47 protein in Escherichia coli, we have cloned the N-terminal part(+1∼+818) of UL47 open reading frame(ORF) into pET14b vector. After transformed the cloned DNA(pET14b-UL47N) into BL21(DE3)pLysS, we identified the UL47 protein was expressed by IPTG(isopropyl β-D-thiogalactopyranoside) induction.