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      • KCI등재

        감귤 과피 가수분해물의 플라보노이드 조성 및 기능적 특성

        이명희(Myung-Hee Lee),허담(Dam Huh),조덕조(DeokJo Jo),이기동(Gee-Dong Lee),윤성란(Sung-Ran Yoon) 한국식품영양과학회 2007 한국식품영양과학회지 Vol.36 No.11

        본 연구에서는 감귤 과피의 Viscozyme L 효소를 첨가하여 가수분해물을 제조하고, 이들의 플라보노이드 조성의 변화를 반응표면분석하여 모니터링하였다. 그 결과 효소처리에 따라 aglycone 형태의 플라노보이드인 hesperetin 및 naringenin 함량이 증가하는 것으로 나타났으며, 가용성 고형분 함량과 aglycone 형태의 플라보노이드 함량이 높은 최적 효소처리 조건은 효소농도 1.5% 및 반응시간 18 hr으로 설정되었다. 일반진피의 경우는 가용성 고형분 함량 48.49% 이고, 플라보노이드는 hesperidin 58.85 ㎎/g만 검출되어졌으나, 최적 효소처리 조건에서는 가용성 고형분 함량은 72.97%로 나타났으며, 플라보노이드 조성은 naringin 1.56㎎/g, hesperidin 31.31 ㎎/g, naringenin 2.58 ㎎/g 및 hesperetin 3.90 ㎎/g으로 각각 나타났다. 일반진피와 효소 처리한 감귤 과피의 전자공여능 및 ACE 저해활성을 측정한 결과 효소 처리한 감귤 과피가 활성이 더 높은 것으로 나타났다. Response surface methodology was employed to investigate the change of flavonoids components of citrus peel hydrolysate using Viscozyme L as the enzyme. As citrus peels were hydrolyzed by the enzyme, hesperetin and naringenin contents of flavonoids aglycone form increased. The optimal enzyme treatment conditions which were superimposed of the maximized levels for soluble solid, hesperetin, and naringenin contents were enzyme concentration of 1.5% and reaction time of 18 hr. In enzyme-untreated citrus peels (CC), soluble solid content was 48.49% and the content of hesperidin only detected flavonoids was 58.85 ㎎/g. In the case of optimal enzyme-treated citrus peels (CE), soluble solid content was 72.97% and the contents of naringin, hesperidin, naringenin and hesperetin were 1.56 ㎎/g, 31.31 ㎎/g, 2.58 ㎎/g and 3.90 ㎎/g, respectively. In the results of electron donating ability and angiotensin converting enzyme inhibition activity, the activity of CE was higher than that of CC.

      • KCI등재

        둥굴레를 첨가한 커피의 관능적 특성 분석

        김혜경(Hye Kyung Kim),정현아(Hyeun-a Jung),안은미(Eun-mi Ahn),허담(Dam Huh),김현철(Hyun Chul Kim),백재은(Jae-Eun Paik) 동아시아식생활학회 2010 동아시아식생활학회지 Vol.20 No.6

        The purpose of this study was to synthesize tasty and healthy solomon's seal coffee by combining solomon's seal and a coffee-making process. Therefore, this study was carried out to establish the most suitable conditions for coffee-making by surface response surface methodology. Using the center synthesis method, we synthesized solomon's seal coffee by considering three factors (solomon's seal, coffee, and temperature) and three levels. The sixteen experimental items for solomon's seal coffee based on surface-reaction analysis were scent (crust break-scent when pouring water in solomon's seal coffee, flavorscent when tasting solomon's seal coffee), taste (clean-cup-pure taste without loose scent, sweetness, and acidity), mouth-feel, aftertaste, overall quality, and other taste degrees. Scores were from 1 to 7, with 7 indicating the highest preference. The optimum mixing rates which meet sensory items were coffee 19.51g, solomon's seal 2.07g, and temperature 90.39℃. Based on the above results, solomon's seal seems to be well suited for coffee. It was also found that solomon's seal has great potential to be well suited with other foods.

      • KCI등재

        연구논문 : 열수추출 및 효소처리에 따른 감귤 과피 추출물의 기능성성분 수득에 대한 연구

        노정은 ( Jung Eun Noh ),윤성란 ( Sung Ran Yoon ),임애경 ( Ae Kyoung Lim ),김혜정 ( Hye Jeong Kim ),허담 ( Dam Huh ),김대익 ( Dae Ik Kim ) 한국식품조리과학회(구.한국조리과학회) 2012 한국식품조리과학회지 Vol.28 No.1

        This study was conducted to investigate the physicochemical properties of citrus peel extracts with different hot water extraction and enzymatic hydrolysis conditions. Enzymatic hydrolysis was also employed using Viscozyme L and results were compared with that of optimized hot water extract. Hot water extraction was performed under different parameters; the sample to solvent ratio(1:20, 1:15, 1:10), extraction time(2, 4 hrs), extraction temperature(85, 95℃) and enzymatic hydrolysis(0, 1%) and the subsequent extracts were used for determining their physicochemical properties, such as total yield, total phenolics, total flavonoids, and electron donating ability (EDA). With the increase in the sample to solvent ratio and extraction time, total yield, total phenolics, total flavonoids and EDA increased. But extraction temperature did not significantly affect the hot water extract. As hot water extract was hydrolyzed by the enzyme, total yield and active ingredients increased rapidly. In the result of total yield, total phenolics, total flavonoids and EDA, the activity of enzyme-treated extract was higher than those of enzyme-untreated extract. Based upon the overall hot water extraction efficiency, it was found that 20 times volume or 120 min at a time at 95℃ after enzyme treatment was optimal.

      • KCI등재

        고지방식이를 섭취한 마우스에서 느티만가닥버섯의 항비만 효과

        류해정(Hae Jeong Ryu),엄민영(Min Young Um),안지윤(Ji Yun Ahn),정창화(Chang Hwa Jung),허담(Dam Huh),김태완(Tae Wan Kim),하태열(Tae Youl Ha) 한국식품영양과학회 2011 한국식품영양과학회지 Vol.40 No.12

        본 연구는 느티만가닥버섯의 항비만 효과를 알아보기 위해 4주령된 C57BL/6 수컷 쥐를 30마리를 1주일간 적응시킨 후, 정상식이군, 고지방식이군, 5% 느티만가닥버섯 분말 첨가 식이군으로 나누어 8주간 실시하였다. 체중 및 체중증가량은 고지방식이군에서 유의적으로 증가하였으며, 느티만가닥버섯 분말 첨가 식이군에서 감소하였다. 간 및 지방조직의 무게도 고지방식이군에 비하여 느티만가닥버섯 처리군에서 낮았다. 고지방 식이에 의한 혈청 중 총콜레스테롤, 인슐린 및 렙틴 수준 증가는 느티만가닥버섯 분말 섭취로 인하여 낮아졌다. 또한, 간 중 지질 함량도 느티만가닥버섯 분말 첨가군에서 감소하였다. HE staining으로 관찰한 간내 지방 축적과 지방세포의 크기도 느티만가닥버섯 분말 첨가식이 군에서 현저히 감소하였다. 부고환 지방조직에서 관찰한 지방생성 관련 유전자인 PPARγ, SREBP-1c, SCD-1의 단백질 발현은 고지방식이군과 비교 시, 느티만가닥버섯 분말 첨가 식이군에서 억제되었다. 이상의 실험결과, 고지방 식이와 함께 공급한 느티만가닥버섯 분말은 체중 감소 및 체지방 축적 억제와 더불어 혈청 지방 수준 개선에도 긍정적 영향을 미치는 것으로 나타났다. This study investigated the possible anti-obesity effects of Hypsizigus marmoreus on high fat-fed mice. Thirty male C57BL/6 mice were randomly divided into 3 groups: a normal diet group (N), a high-fat diet group (HF), and a high-fat diet with 5% Hypsizigus marmoreus group (HF-H). After 8 weeks, the body weights in the HF group significantly increased, while those of the HF-H group decreased. Also, liver and adipose tissue weights in the HF-H group significantly decreased. Total serum cholesterol, leptin, and insulin levels were significantly higher in the HF group than those of the N group, but lower than those of the HF-H group. Accumulation of hepatic lipids was apparent in the HF group, as indicated by HE staining and hepatic lipid analysis, while these effects were improved by supplements with Hypsizigus marmoreus in the HF-H group. Also, a reduction in adipocyte size of the epididymal adipose tissue was observed in the HF-H group. PPARγ, SREBP-1c, and SCD-1 protein expressions were down-regulated in the epididymal adipose tissue of the HF-F group compared to the HF group. Taken together, these results suggest that Hypsizigus marmoreus may an effective anti-obesity treatment.

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