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혼합배지와 지지체 특성에 의한 줄기세포 분화제어: 골연골 조직을 중심으로
한정윤 ( Jeong Yoon Han ),신지원 ( Ji Won Shin ),박소희 ( So Hee Park ),김동화 ( Dong Hwa Kim ),김수향 ( Su Hyang Kim ),강윤경 ( Yun Gyeong Kang ),신정욱 ( Jung Woog Shin ) 한국조직공학과 재생의학회 2011 조직공학과 재생의학 Vol.8 No.2s
This article investigated the potential of substrate characteristics for the control of differentiation of stem cells. For this, two distinctive materials, alginate and HA (hydroxiapatite) contained PCL, were selected. Utilizing rapid prototype (RP) technique we fabricated each type of scaffold. Mesenchymal stem cells (MSCs) derived from New Zealand White rabbits were suspended into alginate solution and the solution was plotted utilizing RP technique. The cocktail media which contain chondrogenic and osteogenic growth factors were used. The basal media were also used for comparison. During 14 days of experiments the samples were harvested four times. The results showed that MSCs tended to be differentiated into osteo- and chondrocyte-like cells when they were on PCL and in alginate scaffold, respectively even both of the two types of scaffolds were in basal media. These findings were more observable when the cocktail media were used. From this research we can stipulate that the characteristics of substrate materials should be considered along with biochemical reagents in the control of differentiation of stem cells. It is also recommended that the biomechanical factors, such as mechanical stimuli, should be considered in view of bio-mimetic environments.
PDMS 표면형상과 간헐적 정수압이 중간엽 줄기세포의 분화 및 증식에 미치는 영향
정재영 ( Jae Young Jeong ),김동화 ( Dong Hwa Kim ),박소희 ( So Hee Park ),신지원 ( Ji Won Shin ),한정윤 ( Jeong Yoon Han ),강윤경 ( Yun Gyeong Kang ),신정욱 ( Jung Woog Shin ) 한국조직공학과 재생의학회 2010 조직공학과 재생의학 Vol.7 No.3
We investigated the effects of PDMS surface patterns on differentiation and proliferation of MSCs along with intermittent hydrostatic pressure (IHP). Also the effect of growth factor was evaluated. For this, we fabricated PDMS substrates with or without patterns. The patterned substrates were made into two types: 5×5×3 μm 3 and 20×20×3 μm 3. The IHP (0.2 MPa, 2 hrs/day) was engaged on the all experimental groups for early 7 days starting 24 hrs after seeding the cells. To examine the cellular responses up to 21 days, we conducted various biological tests: DNA contents, ALP activity and GAG assay, RT-PCR for aggrecan, type II collagen, osteopontin, osteocalcin. The proliferation rates were higher in patterned groups without growth factor. The group with 20 μm pattern showed higher proliferation rate than group with 5 μm pattern. Amounts of GAG normalized by DNA contents in patterned groups were higher than those of non-patterned groups during the experiment periods and were remarkably higher in the groups with growth factor. ALP expressions were so small to be negligible even they tended to be decreased in all groups. activities tended to decrease in all groups. The expressions of chondrogenesis related genes (aggrecan, type II collagen) were observable in patterned groups. Osteopontin, the early marker of osteogenesis, was observable only in the early stage of experiment. Negligible expression of osteocalcin was detected in all groups. From this study, we concluded that patterned surface and IHP have positive effects on proliferation of MSCs and tended to enhance chondrogenesis rather than osteogenesis.