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토끼 할구 융합배의 H-Y 항체에 의한 성감별에 관한 연구 1 . 초기배 절단에 의한 자토 생산
임경순(K . S . Im),최화식(H . S . Choi),정구민(K . M . Chung),양보석(B . S . Yang) 한국축산학회 1992 한국축산학회지 Vol.34 No.3
These experiments were carried out to examine development capacity of bisected blastomere by a simple hand method without micromanipulator from 4-cell to morula stage in rabbit. The results obtained from these experiments are as follows: 1) When rabbit embryos were bisected by hand method, both halves undamaged rate was 37.5(3/8). 65.5(33/52) and 65.2(43/66) in 4-8, 16-cell and morula stage, respectively. 2) When demi embryos were cultured, the percentage of bisected embryos developed into blastocysts was 75, 117 and 123% in 4-8, 16-cell and morula stage. respectively 3) When 30 demi-embryos from 16-cell and 70 demi-embryos from morula were transferred into 3 and 7 recipients, 3/30(10) and 6/70(8.9%) young obtained, respectively.