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DNA 교잡에 의한 토양 미생물 군집의 다양성과 유사성
최영길(Yong Keel Choi),김유영(You Young Kim),송인근(In Geun Song),민병례(Byeong Rye Min),조홍범(Hong Bum Cho) 한국환경생물학회 1999 환경생물 : 환경생물학회지 Vol.17 No.3
To investigate soil bacterial diversity according to vegetation types, directly extracted DNA from 5 different soils were cross-hybridized with each other as a probe and target. Pinus densiflora soil was shown the highest value then agricultured soil>naked soil>grass soil>Quercus mongolicas soil in the order of diversity. Cluster analysis by similarity showed that soil microbial communities were categorized into three groups. [Soil bacterial community, DNA:DNA hybridization, Diversity].
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참나무과 2종 삼림토양의 환경요인 및 효소활성도 비교분석
송인근,최영길 ( In Geun Song,Yong Keel Choi ) 한국환경생물학회 1997 환경생물 : 환경생물학회지 Vol.15 No.2
Physicochemical environmental factors and enzyme activities were compared between two forest soils in which different species of family Quercoideae were present. Two species are Quercus mongolica and Quercus dentata. Soil pH were ranged 6.2∼6.6 in both forest soils. Moisture content and total organic matter were 1.4 and 1.3 times higher in the surface soil of Q. mongolica forest than the other, repectively. Cellulase activities at the surface soils were 4.71 times and 2.1 times higher than 25㎝ depth soils of Q. mongolica and Q. dentata forest, respectively. Between the surface soil of two vegetational forest, cellulase activity of Q. mongolica forest was 1.69 times than the other. Cellulase activity was correlated with the soluble sugar (R^2=0.623). Protease acitivity was 1.4 times higher in surface soil of Q. mongolica than the other. But, it was 10 fold higher in 25㎝ depth lower soil of Q. dentata forest than Q. mongolica. Phosphatase activities were similar in 25㎝ depth soils of two vegetational forest. But, phosphatase including acidic phosphatase (ACPase), neutral phosphatase (NEPase), alkaline phosphatase (ALPase) showed higher activities in surface soil of Q. mongolica forest compared to Q. dentata forest. Among the 3 kinds of pH dependent phosphatases, ACPase showed close correlationship with protease (R^2= 0.659).
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송인근,최영길,최용 漢陽大學校 環境科學硏究所 1994 環境科學論文集 Vol.15 No.-
한국산 건강식품 가공원료인 알로에, 화분, 효모, 효소 가공분말의 초기 미생물오염도는 각각 8.2× 10²cell/g, 8.7 ×??cell/g, 8.8 ×10⁴cell/g, 6.9× 10⁴cell/g으로 측정되었다. 각 시료군별 감마선 조사를 1∼9kGy로 적용한 결과 초기 미생물의 오염도가 가장 낮은 알로에 분말의 경우 3.5kGy에서 완전 살균이 나타났으며 초기 오염도가 가장 높은 화분 가공분말은 7.2kGy정도에서 완전 살균이 가능하였다. 알로에, 화분, 효모 및 효소 가공분말의 선량 적용에 따른 미생물 생존 곡선에서 ??값은 각각 1.30, 1.20,1.27, 1.20으로 산출되었으며 불활성계수(n)가 6일때 7∼8kGy의 치사선량(??)값을 얻었다. The sample used in this experiment was worked material of health foods manufactured in Korea such as aloe, pollens, yeast prepns. and enzyme prepns. The initial microbial population of above food was 8.2 ×10²cell/g-aloe powder, 8.7 ×??cell/g -pollen, 8.8× 10⁴cell/g-yeast prepns, 6.9 ×10⁴cell/g-enzyme prepns. When samples were irradiated as a serial dose from 1kGy to 9kGy using gamma ray-??, aloe powder showed complete sterilization under 3.5kGy dose. In contrast, complete radio-sterilization in pollens is possible around te 7.2kGy dose of ??-gamma irradiation. ?? value(the dose required to eradicate 90% of the population)presented in microbial suvival curve according to serial irradiation dose was calculated 1.30(aloe), 1.20(pollens), 1.27(yeasts prepns.) and 1.20 (Enzymes prepns), respectively. As a inactivation factor(n) was 6, Lethal Dose(??)is 7∼8kGy dose of gamma irradiation.
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