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온도와 질소원 종류에 따른 대형갈조류 감태 (Ecklonia cava)의 생장
최선경,강윤희,박상률 한국환경생물학회 2020 환경생물 : 환경생물학회지 Vol.38 No.3
We investigated the seasonal variations in growth and physiological responses of the kelp species Ecklonia cava to different nitrogen sources to establish indoor culture conditions for mass production. Ecklonia cava was cultivated for 10 days in 16 combinations of seawater temperatures (15, 17, 21, and 25°C) and different nitrogen sources (control; NH-NH4+ 100 μM; NO-NO3- 100 μM; and NHNO-NH4+ 50 μM+NO3-50 μM). The growth and growth rate of the blade were affected by temperature. The mean fresh weight and area-based daily growth rate were the highest (5.8±0.5 and 6.6±0.5% day-1, respectively) at 15°C and the lowest (2.2±0.2 and 3.0±0.3% day-1, respectively) at 25°C. The daily growth rate was the highest in the NH and NO treatments and lowest in the control. The nitrate reductase activity of E. cava varied with water temperature (season). The highest activity was in the control (1.32±0.10 μmol NO2- g-1 dry weight h-1) and the lowest was in the NH treatment (0.25±0.02 μmol NO2- g-1 dry weight h-1). The photosynthetic pigment concentrations reached a maximum value in the NHNO treatment and a minimum value in the control. These results showed that water temperature played an important role in the cultivation of E. cava and that a single supply of NH4+ or NO3- may induce the accelerated growth of E. cava. The growth and physiological responses of E. cava to different nitrogen sources during each season provide valuable information for determining the optimal nitrogen source in E. cava cultivation under indoor conditions. 감태 (Ecklonia cava)는 다년생 대형갈조류로써 활용성이 매우 높은 것으로 알려졌다. 이 연구는 감태의 대량 생산을 위한 실내 배양 조건 확립을 위해서 계절과 질소원 종류에 따른 감태 포자체의 생장과 생화학적 반응을 조사하였다. 제주도 서귀포 해역에 생육하는 감태를 계절별로 채집하여 계절별 수온조건 (봄 17°C, 여름 25°C, 가을 21°C와 겨울 15°C)과 4가지 영양염 조건 [대조구 (control), 100 μM NH4+를 넣은 실험구 (NH), 100 μM NO3-를 추가한 실험구 (NO), 50 μM NH4+와 50 μM NO3-를 함께 넣은 실험구 (NHNO)]에서 배양하여 엽체의 생체량과 면적 변화에 따른 생장률, 질산환원효소 활성도와 광합성 색소 함량을 조사하였다. 감태의 생장은 뚜렷한 계절 변화를 나타냈고, 무게와 면적과의 상관성은 계절별로 차이를 보였다. 무게와 면적의 일일생장률은 겨울에 최고 값 (5.8±0.5와 6.6±0.5% day-1)을 보였고, 여름에 최저 값 (2.2±0.2와 3.0±0.3% day-1)을 나타냈다. 엽체의 일일생장률은 NH와 NO 실험구에서 가장 높았으며 NHNO 실험구에서 중간 값 그리고 대조구에서 가장 낮은 값을 보였다. 감태의 질산환원효소 활성도는 계절적으로 유의한 차이를 나타났으며, 대조구에서 가장 높고 (1.32±0.10 μmol NO2- g-1 dry weight h-1), NH 실험구에서 가장 낮았다 (0.25±0.02 μmol NO2- g-1 dry weight h-1). 엽체 내 광합성 색소의 함량은 대조구에서 가장 낮고 NHNO 실험구에서 가장 높았다. 본 연구결과는 감태 배양을 위한 배양액을 제작 시, 단일 종류의 질소원으로 제작하는 것이 생장률을 향상시킨다는 것을 보여주었으며, 실내배양에 따른 최적 질소원 종류를 결정하는 데 중요한 기초자료를 제공할 것이다.
崔善慶 洌上古典硏究會 2002 열상고전연구 Vol.15 No.-
Hangga "SEODONGYO" which was recorded <king Mu> ( ) part in 『Samgukyusa』, have been studied from the beginning of the gangga research Although the research have been accumulated, it doesn't seems to have much progress about the matters dereved from <king Mu> story. This is mainly caused by differences among researcher's view point, some of them read it as a history, the others as a tale. This study are focused on revealing a new literary interpretation of "SEODONGYO" AND <king Mu> story based on the fact that there have been several problems that should be reviwed closely. As a story <King Mu> part are divided by four sectors : birthmarriage-take a throne-build Miruksa(). The first part of birth story belongs to a typical hero story because Seodong was represented as a son of dragon and also talented. The second part, which can be read as a tricksters' story, Seodong shows his ability by spreading a fake song out to children in order to pursue his own goal. The third part, taking a throne story is regarded as story of fulfillment of one's task. The last part explains Kingmu respects the order of Buddism after he became a king. According to these contexts, "SEODONGYO" also can be treated as a celebration song for the birth of hero, while it had been explained as a love or spell song. "SEODONGYO" alone itself is a part of a myth and shows mrriage between earth mother and heavenly father and birth of heros as result of their marriage. These interpretations reveal possibitities that "SEODONGYO" might had been sung in the ceremonies related with heros. After being derived from the ceremonial place, it could have been handed down mouth to mouth without its original meaning. As a result of these process, this song was understood as merely love song. These unique elements that was shown is "SEODONGYO", presents a high possibility that this song might be originated from ceremonies at the beginning and also give some argumental bases for relation among other Hanggas.