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가토에서 자가유래 골아줄기세포를 이용한 상악동 골 이식술시 비계체로서 Bio-Oss의 효과에 관한 연구
이준(Jun Lee),성대혁(Dae-Hyuk Sung),최재영(Jae-Young Choi),최성림(Sung-Rym Choi),차수련(Su-Ryun Cha),장재덕(Jae-Deog Jang),김은철(Eun-Chul Kim) 대한구강악안면외과학회 2007 대한구강악안면외과학회지 Vol.33 No.5
Mesenchymal stem cells (MSCs) have been though to be multipotent cells that can replicate that have the potential to differentiate into lineages of mesenchymal tissue including the bone, cartilage, fat, tendon, muscle, and marrow stroma. Especially, scaffolds to support cell-based tissue engineering are critical determinants of clinical efforts to regenerate and repair the body. Selection of a matrix carrier imvolves consideration of the matrix’s role as a scaffold for physical support and host tissue integration as well as its ability to support of synergize the osteoinductive program of the implanted mesenchymal stem cell. The aim of this study is to evaluate the effect of autobone and Bio-Oss to adherent mesenchymal stem cells as scaffolds on sinus augmentation with fibrin glue mixture in a rabbit model. 16 New Zealand White rabbits were divided randomly into 4 groups based on their time of sacrifice (1, 2, 4 and 8 weeks). First, mesenchymal stem cells were isolated from iliac crest marrow of rabbits and expanded in vitro. Cell culture was performed in accordance with the technique described by Tsutsumi et al. In the present study, the animals were sacrificed at 1, 2, 4 and 8 weeks after transplantation, and the bone formation ability of each sides was evaluated clinically, radiologically, histologically and histomorphologically. According to the histological observations, autobone scaffolds group showed integrated graft bone with host bone from sinus wall. At 2 and 4 weeks, it showed active newly formed bone and neovascularization. At 8 weeks, lamellae bone was observed in sinus graft material area. Radiologically, autobone with stem cell showed more radiopaque than Bio-Oss scaffolds group. there were significant differences in bone volume between 4 and 8 weeks (p<0.05).
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