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조치흠 ( Chi Heum Cho ),( Hyun Gyo Lee ),( Ji Min Lee ),( So Jin Shin ),( Sang Hoon Kwon ),( Gi Su Lee ),( Chang Ho Song ),( Eun Som Choi ),( Soon Do Cha ) 대한산부인과학회 2015 대한산부인과학회 학술대회 Vol.101 No.-
Objective: The aim of this study was to investigate the anti-proliferative effect of the salinomycin in cell proliferation and apoptosis in primary cultured human uterine leiomyoma cells. Methods: Cell viability was measured by MIT (3-(4 ,5-dimelhyllhiazol-2-yl)-2,S-diphenyltetrazolium bromide) assay. Caspase-3 activity assay and DNA fragmentation assay were performed to determine the effect of apoptosis. The expression of apoptosis regulatory-related proteins was evaluated by western blot. Results: The cell viability and proliferation of uterine leiomyoma cells were significantly reduced by salinomycin treatment in a dose-dependent manner. DNA fragmentation assay results showed apoptotic cell death after salinomycin incubation. Salinomycin activated caspase-3, -8, and -9, causing apoptosis in uterine leiomyoma cells. Down-regulation of Bcl-2, XIAP, and FLIP with a concomitant increase in Bax, Fas, and DR5 were observed. Conclusion: These results provided the first evidence that salinomycin induce both intrinsic and extrinsic apoptosis. Therefore, salinomycin may be a promising chemopreventive and therapeutic agent against human uterine leiomyoma.
조치흠(Chi Heum Cho),차순도(Soon Do Cha),백원기(Won Ki Baek),권건영(Kun Young Kwon),배인수(In Soo Bae) 대한산부인과학회 2001 Obstetrics & Gynecology Science Vol.44 No.5
Objective : Apoptosis or programmed cell death is a normally physiological cell suicide program that is highly conserved among all animals. We previously evaluated overexpression of c-IAP1(Inhibitor of Apoptosis Protein) in ovarian carcinomas compared with normal ovaries. In this study, we demonstrate evidence for the involvement of c-IAP2 in ovarian carcinomas. Methods : Fresh 9 normal ovaries, 5 benign ovarian cysts and 13 ovarian carcinomas were obtained from routine gynecologic surgeries carried out for benign and malignant ovarian tumors. They were examined for the presence of c-IAP2 by RT-PCR(Reverse Transcriptase Polymerase Chain Reaction), Western blot analysis and immunohistochemical stains. Results : Nine of 14 normal and benign ovarian tumors were negative and 11 of 13 ova rian carcinomas were positive for c-IAP2 by RT-PCR. Positive RT-PCR for c-IAP2 was seen in 11/13 of ovarian carcinomas, a significantly higher percentage than in normal and benign ovarian tumors(5/14). All of these tumors showed strong positive for c-IAP2 by western blot and immunohistochemical staining. Whereas negative RT-PCR for c-IAP2 was seen in 9/14 of normal and benign ovarian tumors, a significantly higher percentage than ovarian carcinomas(2/13). Of these 9 negative samples, 6 had positive Western blot and immunohistochemical stains. There was weak concordance of the result. But expression of c-IAP2 in normal ovarian tissue was localized exclusively in the corpus luteum. Therefore, c-IAP2 may play important role in determining the fate of the follicular destiny. There was no expression in normal ovarian stroma cells for c-IAP2. Conclusions : These findings suggest that c-IAP2 is expressed in ovarian carcinomas and emerging role in cancer. The c-IAP2 expression has been investigated in the normal ovary, where apoptosis is thought to play an important role in ovulation.
최신임상강좌 : 생식세포난소종양 (Ovarian Germ Cell Tumors)의 최신 처치 지견
조치흠 ( Chi Heum Cho ) 대한산부인과학회 2005 Obstetrics & Gynecology Science Vol.48 No.2
Significant improvements in the management of ovarian germ-cell tumors have been achieved during the past two decades. The development of more effective chemotherapeutic regimens is clearly the leading cause for improved outcome for these patients. In add
약제 치료에 저항하는 폐 전이 임신성 융모암의 폐절제술을 통한 치험 1 예
이지민(Ji Min Lee),조치흠(Chi Heum Cho),차순도(Soon Do Cha),조상민(Sang Min Cho),권건영(Kun Young Kwon) 대한산부인과학회 2000 Obstetrics & Gynecology Science Vol.43 No.6
Pulmonary metastasis occurs frequently in patients with gestational choriocarcinoma and most of these patients achieve remission with chemotherapy alone. Thus, the indications for surgical intervention are limited, but in appropriately selected patients, resection of a chemotherapy-resistant lung lesion can be curative. We have experienced that pulmonary metastatic choriocarcinoma in a drug-resistant patient was cured by pulmonary resection. So we report this case with a brief review of literatures.
Selective Estrogen Receptor Modulator에 의한 자궁근종의 증식 억제
이민용 ( Min Yong Lee ),조치흠 ( Chi Heum Cho ),권상훈 ( Sang Hoon Kwon ),송대규 ( Dae Kyu Song ),정선욱 ( Sun Wok Chung ),강형옥 ( Hyoung Ok Kang ),윤성도 ( Sung Do Yoon ),차순도 ( Soon Do Cha ) 대한산부인과학회 2004 Obstetrics & Gynecology Science Vol.47 No.6
Objective : Our purpose was to evaluate potential efficacy of selective estrogen receptor modulators (raloxifene and tamoxifen) to human uterine leiomyoma cells. Methods : The samples were collected from ten hysterectomized specimen. we evaluated the estrogen-responsive growth of human uterine leiomyoma and normal myometrial cells. The potential efficacy of Selective Estrogen Receptor Modulators (SERMs: raloxifene and tamosifen) to human uterins leiomyoma cells were conducted by MTS, cell count assay and Western-blot. Results : Human uterine leiomyoma and normal myometrial cells that expressed estrogen receptor (ER) showed increases the cell number in the presence of estrogen compared with ER negative uterine leiomyoma cells. Raloxifene and tamoxifen inhibited estrogen-stimulated proliferation of ER-containing human uterine leiomyoma and normal myometrial cells. Raloxifene was more effective in inhibiting estrogen-induced increases of cell number compared with tamoxifen. Conclusion : The effect of SERMs on leiomyoma was inhibited the cell proliferation without apoptosis or cell cycle arrest. These data suggest that SERM should be examined as candidate of nonsurgical therapeutic agents for uterine leiomyoma.
Resveratrol에 의한 자궁근종세포의 성장억제 효과
강보영 ( Bo Young Kang ),조치흠 ( Chi Heum Cho ),김숙현 ( Sook Hyun Kim ),신소진 ( So Jin Shin ),권상훈 ( Sang Hoon Kwon ),박준철 ( Joon Cheol Park ),이정호 ( Jeong Ho Rhee ),김종인 ( Jong In Kim ),차순도 ( Soon Do Cha ),윤성도 ( 대한산부인과학회 2006 Obstetrics & Gynecology Science Vol.49 No.12
Objective: To examine the effect of resveratrol on cell proliferation and cell cycle progression in the human uterine leiomyoma cells. Methods: MTS [3-(4,5-dimethylthiazol-2-yl)5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium] reduction assay was carried out to determine the viability of human uterine leiomyoma cells. Western blot analysis was done using anti pRB, anti- p21cip1/waf1, anti- p53, anti- cyclin E, anti CDK2 antibodies to detect the presence and expression of these proteins in treatment with resveratrol. DNA fragmentation assay was done to find the rate of apoptosis. Cell cycle analysis for resveratrol treated in human uterine leiomyoma cells was done by FACS (fluorescence-activated cell sorter) analysis. Results: Resveratrol induced growth inhibition in a dose dependent manner, treatment with 100 μM/L resveratrol blocked 30% cell growth. From Western blot analysis it revealed resveratrol induced the expression of p53 increasing. Caspase pathway was activated and cleavage of PARP was occurred. Apoptosis took place but in a reduced manner. FACS results showed that resveratrol increased the percentage of cells in sub G1 phase. Conclusion: Resveratrol, a dietry phytoalexin, inhibited cell proliferation and induced cell cycle arrest at sub G1 by enhancing the production of p53. These results indicate that resveratrol will be a promising agent chemopreventives or therapeutics against human uterine leiomyoma cells.