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趙三增,金鎭漢 충북대학교 농업과학기술연구소 1990 農業科學硏究 Vol.8 No.1
This study was conducted to evaluate several factors, such as media, plant growth regulators, parts of peduncle and varieties, which affect callus growth and organogenesis in tissue culture. The results obtained were summarized as follows ; 1.Callus formation was higher than 95% in all treatments, while Murashige and Skoog(MS) medium induced good result in organogenesis. 2.Higher ratio of adventitious roots was developed at higher 2, 4- D (2, 4-Dichlorophenoxy acetic acid) concentration in B5 (Gamborg et al.) medium. 3.Premeristem tissue were formed profusely when segments of auxillary bud tissue were cultured on MS medium containing 1.0/mg/1 NAA (α-Naphthalene-acetic acid) and 2.0 mg/1 BA(6-Benzylaminopurine). 4.Comparing to the single application of NAA combined treatment of NAA and BA in MS medium decreased adventitious roots, but increased shoot differentiation. 5.Organogenesis was higher in order of Top > Base > Middle parts of peduncle. 6.Variety 'Okina', which grew vigorously, showed greater rate of callus growth and organogenesis than variety 'Ib-chu'.