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Vibrio parahaemolyticus의 분리 및 그 M.I.C.에 대한 고찰
오흥백 ( Hung Baek Oh ),조명원 ( Myung Won Jo ) 대한임상검사과학회 1979 대한임상검사과학회지(KJCLS) Vol.11 No.1
1. Vibrio Parahaemolyticus was first isolated from a victim of food poisoning by Dr. Fujino, in 1951. It has been reported that about 50-70per cent of the cases of bacterial food poisoning in Japan have been caused V. parahaemolyticus, mostly Prevalent between june and September. 2. Of total 16 strains isolated, 15 strains were from 21 food poisoning patients and 1 strain from salt dressed crab. 3. All of the rectal swabs were streaked`` on T.C.B.S. (Thiosulfate Citrate Bile salts Sucrose) agar plates, and enriched in to 1% peptone water with 3% sodium chloride. On T.C.B.S. agar plate, the colonies showed bluish green color and smooth withmedium size, and no vibrio colony was found on MacConkey, S.S. and blood agar plates. The biological characteristics and phisiological properties were shown in Table 4. 4. All of V. parahaemolyticus were highly susceptible to chloramphenicol and tetracycline group antibiotics (oxytetracycline and Minocin) by the dilution method with of M.I.C. 2mcg/ml. or less. They were resistant at 16-356mcg/ml. to penicillin group antibiotics (Ampicillin and sulbenicillin), and highly resistant to aminoglycoside group antibiotics (kanamycin, Streptomycin and Dibekacin) at M.I.C. 32mcg/ml. or more, while slightly to gntamycin with M.I.C. of 8mcg/ml. or more. They were also moderately resistant to cephalosporin antibiotic with M.I.C. of 32mcg/ml. or more. 5. Of total 16 strains isolated, 7 strains of K-3, 4 strains of K-18, 2 strains of K-22, 1 strain of K-56 and unty peable 2 strains were detected. Although untypeable 2 strains were not agglutinated with any K-type multiple serum , their biological characteristics were same as that of the typical Vibrio parahaemolyticus. It suggests that these 2 strains would be new K type.
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