http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
정행선 ( Haeng Sun Jung ),이수현 ( Su Hyon Lee ),류양환 ( Yang Hwan Ryu ),정경미 ( Kyoung Mi Jung ),김채욱 ( Chae Wook Kim ),임경민 ( Kyung Min Lim ),손영숙 ( Young Sook Son ) 한국동물실험대체법학회 2009 동물실험대체법학회지 Vol.3 No.1
Alternative methods to the Draize eye irritation test, such as the BCOP, HET-CAM, ICE, and IRE, are used to evaluate the ocular irritation potential of cosmetic, livelihood articles or industrial chemicals. In order to improve the sensitivity and specificity of alternative eye irritation test, we developed a three-dimensional human corneal model that uses normal human corneal epithelial cells. Our corneal tissue model consists of normal human corneal epithelial cells on cell culture inserts at the air liquid interface, differentiating to form a stratified, squamous epithelium similar to that found in the human cornea, exhibiting in vivo like morphological and histological characteristics. In this study, two laboratories have tested 20 reference chemicals using the same study protocol. The results were compared to in vivo data as well as previously published data obtained in the other three-dimensional corneal model test. Although an overall accuracy of 85% was obtained (sensitivity=100% and specificity=67%), further experiments are required to confirm and validate these preliminary results.
정행선 ( Haeng Sun Jung ),이수현 ( Su Hyon Lee ),류양환 ( Yang Hwan Ryu ),정경미 ( Kyoung Mi Jung ),김채욱 ( Chae Wook Kim ),임경민 ( Kyung Min Lim ),손영숙 ( Young Sook Son ) 한국동물실험대체법학회 2009 동물실험대체법학회지 Vol.3 No.1
Alternative methods to the Draize eye irritation test, such as the BCOP, HET-CAM, ICE, and IRE, are used to evaluate the ocular irritation potential of cosmetic, livelihood articles or industrial chemicals. In order to improve the sensitivity and specificity of alternative eye irritation test, we developed a three-dimensional human corneal model that uses normal human corneal epithelial cells. Our corneal tissue model consists of normal human corneal epithelial cells on cell culture inserts at the air liquid interface, differentiating to form a stratified, squamous epithelium similar to that found in the human cornea, exhibiting in vivo like morphological and histological characteristics. In this study, two laboratories have tested 20 reference chemicals using the same study protocol. The results were compared to in vivo data as well as previously published data obtained in the other three-dimensional corneal model test. Although an overall accuracy of 85% was obtained (sensitivity=100% and specificity=67%), further experiments are required to confirm and validate these preliminary results.
인공피부모델 KeraSkinTM을 이용한 유전독성 평가
이수현 ( Su-hyon Lee ),정행선 ( Haeng-sun Jung ),김설영 ( Seol-yeong Kim ),김혜수 ( Hye Soo Kim ),임경민 ( Kyung-min Lim ),정영신 ( Young-shin Chung ),최태부 ( Tae-boo Choe ) 대한화장품학회 2016 대한화장품학회지 Vol.42 No.3
소핵시험은 세포분열 단계 중 간기 세포의 세포질 내 소핵 유무를 조사함으로써 유전독성을 평가하는 시험법이다. 최근 화장품 안전성 평가에 동물실험을 금지하거나 최소화하려는 노력이 확산되고 있어 유전독성 평가에 있어서도 기존의 동물실험이 아닌 새로운 in vitro 시험법이 요구되고 있다. 본 연구에서는 3차원 배양인공피부모델인 KeraSkin<sup>TM</sup>을 이용하여 도포 처치된 물질의 유전독성을 평가하였다. 2종의 유전독성물질인 mitomycin C (MMC)와 methyl methanesulfonate (MMS)는 농도 의존적으로 세포독성과 소핵 형성이 유도된 반면, 대조물질인 4-nitrophenol (4-NP)와 trichloroethylene (TCE)에서는 농도 의존적으로 세포독성은 관찰되었으나 소핵은 형성되지 않았다. 따라서 인공피부모델을 이용한 소핵시험이 화장품과 같은 피부적용물질의 in vitro 유전독성 평가에 유용할 것으로 사료된다. Micronucleus test is genotoxicity assay for detection of micronuclei in the cytoplasm of interphase cells. The reduction and replacement of in vivo toxicity testing on animals require the development of in vitro models to predict the genotoxicity or other tests for cosmetic products. In this study, we evaluated a genotoxicity assay for topically applied chemicals using a three-dimensional human reconstructed skin model, KeraSkinTM. Two genotoxins, mitomycin C (MMC) and methyl methanesulfonate (MMS), induced significant dose-related increases in cytotoxicity and micronuclei induction in the skin model. In contrast, two non-genotoxins, 4-nitrophenol (4-NP) and trichloroethylene (TCE), induced cytotoxicity but not micronucleus formation. In conclusion, micronucleus test using human skin model may be useful for predicting in vitro genotoxic potentials of cosmetic products.
장원희 ( Won Hee Jang ),정경미 ( Kyoung Mi Jung ),최선경 ( Sun Kyung Choi ),정행선 ( Haeng Sun Jung ),이수현 ( Su Hyon Lee ),최영진 ( Young Jin Choi ),박영호 ( Young Ho Park ),임경민 ( Kyung Min Lim ) 한국동물실험대체법학회 2011 동물실험대체법학회지 Vol.5 No.1
Dry skin is induced by excessive transdermal water loss due to the damages of intracellular lipids. Moisturizer is safe and effective for improving dry skin, however, there is few efficient alternative method to evaluate moisturizing activity of new cosmetic ingredients. Here, we investigated the moisturizing activity of a face powder coated with mannosylerithritol lipids (MEL) that are similar to ceramides in structure and intermediates in the biosynthesis of intracellular lipids, without using animals or human but Keraskin™, a three-dimensional reconstructed human epidermis. After Keraskin™ was incubated, MEL-coated sericite or uncoated sericite was applied and then sodium lauryl sulphate (SLS) was treated with a nylon mesh covering to spread it uniformly. After incubation for 15 min, Keraskin™ was washed and it was post-incubated for 24 hr. At the end of post-incubation, Keraskin™ was incubated with MTT for 3 hr to measure viability. Formed purple formazan was extracted with isopropanol for 2 hr. Absorbance of extract(A590) was measured using spectrophotometer. Cell viability was calculated by dividing with the absorbance of negative control. The viability of reconstructed epidermis treated 10% SLS was determined to be 12.3 ± 4.87%. When SLS was co-treated with sericite or MEL-coated sericite, cell viability was increased to 23.7 ± 2.92% or 71.5 ± 20% (p<0.05), respectively. MEL-coated sericite inhibited SLS-induced cytotoxicity substantially in comparison with sericite alone. Based on these results, reconstructed human epidermis can be used for the evaluation of the efficacy of cosmetic ingredients or products.
연구논문 : 인공피부모델을 이용한 보습파우더의 보습 효능 평가
장원희 ( Won Hee Jang ),정경미 ( Kyoung Mi Jung ),최선경 ( Sun Kyung Choi ),정행선 ( Haeng Sun Jung ),이수현 ( Su Hyon Lee ),최영진 ( Young Jin Choi ),박영호 ( Young Ho Park ),임경민 ( Kyung Min Lim ) 한국동물실험대체법학회 2011 동물실험대체법학회지 Vol.5 No.1
Dry skin is induced by excessive transdermal water loss due to the damages of intracellular lipids. Moisturizer is safe and effective for improving dry skin, however, there is few efficient alternative method to evaluate moisturizing activity of new cosmetic ingredients. Here, we investigated the moisturizing activity of a face powder coated with mannosylerithritol lipids (MEL) that are similar to ceramides in structure and intermediates in the biosynthesis of intracellular lipids, without using animals or human but Keraskin™, a three-dimensional reconstructed human epidermis. After Keraskin™ was incubated, MEL-coated sericite or uncoated sericite was applied and then sodium lauryl sulphate (SLS) was treated with a nylon mesh covering to spread it uniformly. After incubation for 15 min, Keraskin™ was washed and it was post-incubated for 24 hr. At the end of post-incubation, Keraskin™ was incubated with MTT for 3 hr to measure viability. Formed purple formazan was extracted with isopropanol for 2 hr. Absorbance of extract(A590) was measured using spectrophotometer. Cell viability was calculated by dividing with the absorbance of negative control. The viability of reconstructed epidermis treated 10% SLS was determined to be 12.3 ± 4.87%. When SLS was co-treated with sericite or MEL-coated sericite, cell viability was increased to 23.7 ± 2.92% or 71.5 ± 20% (p<0.05), respectively. MEL-coated sericite inhibited SLS-induced cytotoxicity substantially in comparison with sericite alone. Based on these results, reconstructed human epidermis can be used for the evaluation of the efficacy of cosmetic ingredients or products.
연구논문 : 인공피부모델 KeraSkin(TM) 피부자극성 시험 대체시험법의 실험실간 검증
문지영 ( Ji Young Moon ),이수현 ( Su Hyon Lee ),정행선 ( Haeng Sun Jung ),류양환 ( Yang Hwan Ryu ),정경미 ( Kyoung Mi Jung ),임경민 ( Kyung Min Lim ),박철범 ( Cheol Beom Park ) 한국동물실험대체법학회 2010 동물실험대체법학회지 Vol.4 No.2
Reconstructed skin provides advantages to single cell culture testing and leads to promising results as shown by different validation studies. In this study we investigated the interlaboratory validation for the efficacy of “Keraskin(TM)” model. We conducted endpoint analysis including cell viability (MTT reduction) and IL-1α release. Eighteen item, which were ECVAM test meterial, were selected for this validation study. Results of MTT assay were not different between EPI-200 and Keraskin(TM), but IL-1α levels of some test items different between EPI-200 and Keraskin(TM). We concluded that this Keraskin(TM) model is useful for alternative skin irritation test, although other tests should be conducted for many other chemicals in international validation study.
인공피부모델 KeraSkin(TM) 피부자극성 시험 대체시험법의 실험실간 검증
문지영 ( Ji Young Moon ),이수현 ( Su Hyon Lee ),정행선 ( Haeng Sun Jung ),류양환 ( Yang Hwan Ryu ),정경미 ( Kyoung Mi Jung ),임경민 ( Kyung Min Lim ),박철범 ( Cheol Beom Park ) 한국동물실험대체법학회 2010 동물실험대체법학회지 Vol.4 No.2
Reconstructed skin provides advantages to single cell culture testing and leads to promising results as shown by different validation studies. In this study we investigated the interlaboratory validation for the efficacy of “Keraskin(TM)” model. We conducted endpoint analysis including cell viability (MTT reduction) and IL-1α release. Eighteen item, which were ECVAM test meterial, were selected for this validation study. Results of MTT assay were not different between EPI-200 and Keraskin(TM), but IL-1α levels of some test items different between EPI-200 and Keraskin(TM). We concluded that this Keraskin(TM) model is useful for alternative skin irritation test, although other tests should be conducted for many other chemicals in international validation study.