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열처리한 자가혈청이 성인 골수간엽줄기세포의 증식과 조골세포 분화에 미치는 영향
박의균 ( Eui Kyun Park ),이선영 ( Sun Young Lee ),김태호 ( Tae Ho Kim ),정필훈 ( Phil Hoon Choung ),강길선 ( Gil Son Khang ),손영숙 ( Young Sook Son ),김석영 ( Suk Young Kim ),김신윤 ( Shin Yoon Kim ) 한국조직공학과 재생의학회 2005 조직공학과 재생의학 Vol.2 No.1
Tissue engineering using mesenchymal stem cells (MSCs) is a promising technology for treatment or regeneration of tissue defects. However, to put this technology into practical use, it is necessary to determine suitable biomaterials as well as to expand MSCs ex vivo. One major challenge of ex vivo expansion of MSCs is to avoid the use of fetal bovine serum (FBS), which is a fundamental culture supplement. In this study, we investigated effects of autologous serum on proliferation and osteoblastic differentiation of bone marrow stem cells (BMSCs) isolated from patients with skeletal diseases. We isolated BMSCs from 7 independent patients with avasular necrosis (AVN; 3 cases), osteoarthritis (OA; 2 cases), femoral head fracture (1 case) or hip dysplasia (1 case) and compared the effects of FBS, heat inactivated (HAS) and normal autologous serum (HA) on the proliferation of BMSCs. Proliferation analyses of BMSCs revealed that 5 out of 7 BMSCs cultured in 10 % HAS showed higher proliferation than cells cultured in 10 % FBS. Moreover, analysis of osteoblastic differentiation as assessed by alkaline phosphatase (ALP) staining, a marker for osteoblastic differentiation, showed that HAS, AS and FBS were capable of supporting osteoblastic differentiation of BMSCs at the similar level. Interestingly, mineralization was increased in BMSCs cultured in HAS (5 out of 6 BMSCs). These results demonstrate that HAS compared with conventional FBS has a better potential to stimulate proliferation and mineralization of BMSCs as well as to support their osteoblastic differentiation.
자가혈청하에서 FGF-2와 덱사메타손에 의한 골수중간엽줄기세포의 증식과 분화에 대한 효과
손민정 ( Min Jung Shon ),이선영 ( Sun Young Lee ),김태호 ( Tae Ho Kim ),유창국 ( Chang Kook You ),김석영 ( Suk Young Kim ),정필훈 ( Phil Hoon Choung ),손영숙 ( Young Sook Son ),박의균 ( Eui Kyun Park ),김신윤 ( Shin Yoon Kim ) 한국조직공학과 재생의학회 2005 조직공학과 재생의학 Vol.2 No.4
Previously we have shown that heat-inactivated autologous serum (HAS) has a potential to stimulate proliferation and ostoblastic differentiation of bone marrow stromal cells (BMSCs). In the present study we investigated whether stimulatory effects of HAS on proliferation and osteoblastic differentiation of BMSCs are further potentiated by fibroblast growth factor-2 (FGF-2) and dexamethasone (Dex). As expected, FGF-2 and Dex stimulated proliferation of BMSCs up to 17% at 5 days and 26% at 7 days of culture compared to HAS control. These results suggest that FGF-2 and Dex in the presence of HAS further stimulate proliferation of BMSCs. In order to examine whether BMSCs expanded with FGF-2, Dex and HAS harbor multipotency, the expanded cells were stimulated with either osteogenic or adipogenic cocktails. BMSCs expanded with FGF-2, Dex and HAS for 7 days were able to be differentiated into either osteoblasts or adipocytes. Taken together, these results demonstrate that FGF-2 and Dex in combination with HAS further stimulates proliferation of BMSCs and these expanded cells maintain potentials to be differentiated into either osteoblasts or adipocytes.