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김현정 ( Hyun Jeong Kim ),황래홍 ( Lae Hwong Hwang ),정지헌 ( Ji Heon Joeng ),윤은선 ( En Sun Yoon ),박노운 ( Noh Woon Park ),한인규 ( In Gyou Han ) 한국가축위생학회 1999 韓國家畜衛生學會誌 Vol.21 No.3
A method for the determination of noboviocin in milk was presented by high performance liquid chromatography(HPLC). The novobiocin in the spiked sample was extracted with methanol and evaporated under vacuum. After evaporating, the residue was mixed with distilled water for 2ml, filtrates with 0.45um acrodisc was injected into HPLC. The results obtained were as follows; 1. The calibration curve of novobiocin was showed constantly linear(r 0.999) in the range of 100~500ng/ml. 2. The mean recovery rate of novobiocin from the spiked milk sample were 88~98%. 3. The coefficients of variation were 2.6~5.8%. 4. The lowest detectable limit of novobiocin was 25ppb.