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은사시의 根頭癌腫에서 分離한 Agrobacterium tumefaciens에 關한 硏究
全桓惠,張茂雄 嶺南大學校 環境問題硏究所 1985 環境硏究 Vol.5 No.2
Agrobacterium tumefaciens (YPI, YPII, YPIII, YPIV, YPV) isolated from crown galls of Korean native populus tomentiglandulosa had characteristics as follows. Korean native strains appeared to be gram negative rod form having peritrichous flagella. Plasmids were isolated from YP strains by the alkaline method. The electrophoresis was performed with the plasmid band in 0.7% agarose gel. The experiment indicated that YPI had one plasmid, YPII had 5 plasmids, but YPIII, YPIV, YPV had no plasmid. The crude plasmids isolated from YPI and YPII strains were purified by CsCI-Et.Br density gradient centrifugation. The purified plasmids revealed open and supercoiled circular molecules under electron microscope. In electron microscopy of the ultra-thin sections of crown gall tissues, it was shown that bacterial cells were in the cytoplasm of crown gall cells and were surrounded by the membrane