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Di(2-ethylhexyl) phthalate에 의해 유도된 DNA 손상과 소핵 형성
김종원(Jong Won Kim),한의식(Eui Sik Han),박미선(Hai Kwan Jung),엄미옥(Mi Sun Park),김인숙(Mi Ok Eom),전혜승(In Sook Kim),정해관(Hye Seung Jun),심웅섭(Woong Seop Sim),오혜영(Hye Young Oh) 한국환경성돌연변이발암원학회 2001 한국환경성돌연변이·발암원학회지 Vol.21 No.1
Di-2-ethylhexyl phthalate (DEHP) is the most commonly used phthalate ester in polyvinyl chloride formulations including food packing and storage of human blood. DEHP is a well known as non-genotoxic carcinogen and endocrine disrupting chemical (EDC). DEHP have shown all negative results in ICH-guildeline recommended standard genotoxicity test battery. In this study, to assess the clastogenic and DNA damaging effect in human-derived tissue specific cells, DEHP was treated in human derived MCF-7 cells, HepG2 cells, LNCap cells, BeWo cells, MCF-10A cells, and female peripheral blood cells using micronucleus assay and in human<br/> breast carcinoma MCF-7 cells up to 1.28×10-²M using Comet assay. The in vitro micronucleus assay is a mutagenicity test system for the detection of chemicals which induce the formation of small membrane bound DNA fragment i.e. micronuclei in the cytoplasm of interphase cells, originated from clastogenic and/or aneugenic<br/> mechanism. The single cell gel electrophoresis assay (Comet assay) is used to detect DNA strand-breaks and alkaline labile site. In our results, DEHP increased significantly and/or dose-depentently and time-dependently micronucleus frequency at the 6 and 24 hr without metabolic activation system only in MCF-7 cells. DEHP treated with 2 hrs in MCF-7 cells using Comet assay induced DNA damage dose-depentantly.
Syrian hamster embryo 세포와 mouse embryo BalB/c 3T3 세포에서의 bisphenol A의 세포 형질전환 연구
김종원(Jong Won Kim),한의식(Eui Sik Han),박미선(Mi Sun Park),엄미옥(Mi Ok Eom),전혜승(Hye Seung Jun),민수진(Su Jin Min),김인숙(In Sook Kim),정해관(Hai Kwan Jung),심웅섭(Woong Seop Sim),오혜영(Hye Young Oh) 한국환경성돌연변이발암원학회 2001 한국환경성돌연변이·발암원학회지 Vol.21 No.1
To identify nongenotoxic carcinogen determined as negative by ICH guideline-recommended standard genotoxicity test battery; Ames test, chromosome aberrration assay, mouse lymphoma tk+/- assay, in vivo micronucleus assay, we picked bisphenol A as a model compound. In this study, we applied in vitro BalB/c 3T3 cell transformation assay and Syrian hamster embryo (SHE) cell transformation assay. Bisphenol A was treated upto 769.2 ug/ml in BalB/c 3T3 cells and upto 125 ug/ml in SHE cells. bisphenol A didn't induced morphological transformation both with one stage treatment protocol and with two stage treatement protocol. But, treated for 48 hr, Bisphenol A induced morphological transformation significantly in SHE cells.