안성천산 (安城川産) 흰줄납줄개의 난발생과 (卵發生) 자어의 (仔魚) 발육 (發育) 및 자어의 (仔魚) 표피상돌기에 (表皮上突起) 관하여

영목신양 (鈴木伸洋),전상린 ( Nobuhiro Suzuki,Sang Rin Jeon ) 한국하천호수학회 1988 생태와 환경 Vol.21 No.1

The development of eggs and larvae and minute tubercles on the skin surface in larvae of Rhodeus ocellatus from Anso^˘ng-river, Korea were observed. The spawning period extended from the mid of April to the beginning of August. The unfertilized egg is pear in shape, measuring 2.61 -2.75 mm in long axis and 1.23-1.35 mm in short axis, opaque yellow in color. The number of ripe eggs obtained ranged from 27 to 34 per on spawning. The eggs began to hatch approximately 39 hours after insemination and larvae, attained 8.1 mm in TL, reached free swimming stage 23 days after hatching at water temperature of 22± 1 ℃ . The larvae started to practice reeding on commerical diets. The egg and larval development and minute tubercles on the skin surface in larvae of this form were similar to those of R. ocellatus smithi and R. ocellatus ocellatus from Japan. However, this form was characterized by following characters. The time required for each embryonic and larval stage in this form were equivalent to those of R. ocellatus smithi, while this form shared the morphological characters, which are the presence of whitish parts on the first. ventral rays and the number of pored scales on lateral line system, with R. ocellatus ocellatus.

줄납자루의 난발생 (卵發生)·자어의 (仔魚) 발육 (發育) 및 자어의 (仔魚) 표피상돌기와 (表皮上突起) 인두치 (咽頭齒) 저작돌기의 (咀嚼突起) 형태

영목신양 (鈴木伸洋),전상린 ( Nobuhiro Suzuki,Sang Rin Jeon ) 한국하천호수학회 1987 생태와 환경 Vol.20 No.4

The development of eggs and larvae and minute tuvercles on the skin surface in larvae, and the pharyngeal apparatus of Acheilognathus yamatsutae were observed. The egg began to hatch approximately 39 hours after insemination and the larvae reached free-swimming stage 24 days after hatching at water temperature of 22±1℃. The egg and larval development and minute tubercles in larvae of this species relatively similar to those of two subspecies A. tabira tabira and A. tabira subsp. (a). However, this species is characterized in larval development by the following characters: the dorsal part of the yolk sac is slightly developed to form a pair of hilly projections, and minute tubercles on the skin surface of posterior parts of both the yolk sac and the body are hemispheric in shape. Moreover, in the morphological characters of pharyngeal apparatus, the occlusal grooves on the pharyngeal teeth were relatively developed and the chewing area on the first tooth was narrow, and the masticatory process of the basioccipital bone is ovoid-shaped and slightly depressed anteriorly. These characters are considered that show clear the phylogenetic ones between Group 1 and Group 2, suggesting phylogenetic relationship among acheilognathine fishes, exhibited by Suzuki and Hibiya (1985).

납줄갱이의 난발생 (卵發生) , 자어의 (仔魚) 발육 (發育) 및 자어의 (仔魚) 표피상돌기 (表皮上突起)

영목신양 (鈴木伸洋),전상린 ( Nobuhiro Suzuki,Sang Rin Jeon ) 한국하천호수학회 1988 생태와 환경 Vol.21 No.4

The egg and larval development of Rhodeus suigensis of two localities from Korea were observed under the controlled water temperature at 22± 1 ℃ . The eggs of this species in each locality began to hatch about 43 hours after insemination and those larvae reached the free-swimming stage about 20 days after hatching. As regards the comparative development of egg and larval stage in each locality is approximately equal except for a downward projection of the anteriormost part of yolk sac is more intensively developed in Palan-river than in Ansong-river. Moreover, no differences is found in the egg and larval development between R. suigensis from Korea and this species from Japan except for the former reached the free-swimming stage earlier than the latter. In addition, the egg and larval development and minute tubercles on the skin surface of larvae among R. suigensis from Korea and both subspecies of R. atremius and R. suigensis from Japan resemble each other. Uchida`s description for the development of R. notatus is very similar to those of R. atremius and R. suigensis. From these facts, three species of R. atremius, R. suigensis and R. notatus are considered to be separated at subspecies level.

한국 (韓國) 및 일본산 (日本産) 칼납자루의 난발생 (卵發生) , 자어의 (仔魚) 발육 (發育) , 자어의 (仔魚) 표피상돌기 (表皮上突起) 및 이들의 교배에 관하여

영목신양 (鈴木伸洋),전상린 ( Nobuhiro Suzuki,Sang Rin Jeon ) 한국하천호수학회 1988 생태와 환경 Vol.21 No.4

The development of eggs larvae and minute tubercles on the skin surface of larvae of Acheilognathus limbata from two localities of Somjin-river and Kum-river in Korea were observed. The egg began to hatch approximately 45 hours after insemination and the larvae reached free-swimming stage about 22 days after hatching at water temperature of 22± 1 ℃ . A. limbata in Korea shared the following characters, which are shared by A. limbata in Japan, A. lanceolata, A. signifer and Tanakia tanago, i.e., (1) a pair of hilly projections on the yolk sac and circular cone-like minute tubercles and scale-like tubercles on part (a) and hemispheric minute tubercles on part (b), and vestigial minute tubercles on part (c) of the body. These facts suggest that they are considered to be closely related species, because these characters are the important marks for determining phylogenetic relationships. However, A. limbata in Korea was distinguishable in egg and larval development from A. limbata in Japan, A. lanceolata, A. signifer and T. tanago by the following characters: (1) ripe eggs are nearly spindly shape (2) embryonic development is faster (3) anteriormost part of yolk sac elongates forward to form a projection with a convex yolk in newly hatched larvae. Moreover, the crossings of A. limbata between in Korea and in Japan were not satisfactory and no hatched larvae obtained. These facts suggest that at least A. limbata from both localities of Somjin-river and of Kum-river in Korea should be separated at species level from A. limbata in Japan.

묵납자루의 난발생과 (卵發生) 자어의 (仔魚) 발육 (發育) 및 자어의 (仔魚) 표피상돌기 (表皮上突起)

영목신양 (鈴木伸洋),전상린 ( Nobuhiro Suzuki,Sang Rin Jeon ) 한국하천호수학회 1988 생태와 환경 Vol.21 No.3

The development of eggs and larvae and minute tubercles on the skin surface of larvae of Acheilognathus signifer in the two localities of Imjin-river and Namhan-river were observed. The egg began to hatch approximately 53 hours after insemination and the larvae reached free-swimming stage about 20 days after hatching at water temperature of 22±1 ℃. The egg and larval development and minute tubercles on the skin surface in larvae of this species were similar to those of A. lanceolata, A. limbata and T. tanago distributed in Japan, However, A. signifer was distinguishable in the egg and larval development from A. lanceoleta and A, limbata by the following characters; the perivitelline space is narrow, embryonic and larval development is faster, and the miunte tubercles on the skin surface of anteriormost part of yolk sac in newly hatched larvae is hemispheric in shape. These characters of A. signifer is shared with T. tanago. As regards larval development, it is considered that A. signifer is more resemblance to T. tanago than A. limbata and A. lanceolata.

납줄갱이와 각시붕어의 인공교잡실험에 관하여

영목신양 (鈴木愼洋),전상린 ( Nobuhiro Suzuki,Sang Rin Jeon ) 한국하천호수학회 1988 생태와 환경 Vol.21 No.2

Acheilognathus limbata , 칼납자루 , 흰줄납줄개 , 납줄갱이의 암컷과 묵납자루 수컷 사이의 교배실험

영목신양 (鈴木伸洋),전상린 ( Nobuhiro Suzuki,Sang Rin Jeon ) 한국하천호수학회 1994 생태와 환경 Vol.27 No.4

Hybridization experiments between females of four species of Acheilognathus limbata, A. koreensis, Rhodeus ocellatus, and R. suigensis and male of A. signifer was carried under controlled water temperature of 22±1˚C. The hybrids between females of three species of Acheilognathus limbata, Rhodeus ocellatus and R. suigensis and male A. signifer were survived to adulthood. The morphological characters of these hybrids were generally intermediate between the parental species. The crossing from female A. limbata and male A. signifer produced the F_1, and F_2 hybrids. The hybrids between females of two species of R. ocellatus and R. suigensis and male A. signifer were sexually male but they were sterile because of abnormal germ cells occasioned by phagocytosis of spermatocytes. No eggs bred from female A. koreensis and male A. signifer survived beyond the blastopore stage nearly closed stage.

Development of the Bitterling, Acheilognathus rhombeus(Cyprinidae) from Korea.

Suzuki, Nobuhiro,Jeon, Sang Rin 상명대학교 기초과학연구소 1991 기초과학연구 Vol.5 No.-

江原道鐵原郡의 漢灘江에서 採集한 납지리의 個體發生을 22℃의 水溫飼育下에서 經詩的으로 觀察하였다. 本種의 卵은 鷄卵形으로 제리狀인 物質에 덮여 있으며 1回에 平均19粒의 卵이 搾出되었다. 卵發生 및 仔魚의 發育形態는 Nakamura(1969)가 記載한 日本産의 것과 매우 닮았다. 特히 仔魚는 S子狀으로 몸을 꿈틀거리는 듯한 運動을 하는데 이로 미루어 볼때 납지리 Acheilognathus rhombeus는 日本産인 A. typus와 A. longipinnis, 그리고 韓半島産인 큰납지리 Acanthorhodeus asmussi와 가시납지리 Acan. gracilis와 近緣關係에 있음이 示唆되었다. 本種은 韓半島産 납자루類中에서는 唯一하게 秋季産卵型으로 仔魚의 發育이 一定한 段階에서 遲延되었다. 이러한 發育의 遲延은 遺傳的인 支配를 받으며 水溫低下等의 環境要因에 依하지 않는다는 事實이 밝혀졌다. 그리고 이 現象은 秋季産卵型의 適應形質이라고 解釋되었다. 또한 仔魚의 S字狀運動은 日本産인 납자루類에서는 秋季産卵型에 出現하는 形質인데 韓半島産에서는 큰납지리와 가시납지리처럼 春季産卵型에도 出現하는 形質이다. 이러한 事實로부터 系統發生은 큰납지리나 가시납지리 같은 種들로부터 秋季産卵型인 種이 出現하였음이 示唆되어진다. The development of eggs and larvae in Acheilognathus rhombeus from Korea were observed. As regards the morphological characters of the egg and larval developnemt, these from Korea are closely similar to those from Japan. For the duration of all larval stages, A. rhombeus larvae share the character such as the incessant wiggly move ment pattern as that of fly maggot with the larvae of A. typus, A. longipinnis, Acanthorhodeus asmussi and Acan. gracilis. From this fact, it should be supposed that A. rhombeus is closely related to these species in the phylogenetic relationships. This species is the only autumun-spawning bitterling in Korea and the larval growth always retards in such larval stage as the duration from B to D in Suzuki and Hibiya's (1985) developmental stage, of which the larval satge shows no appearence of melanophores on the body. The heritable retardation is a adaptation for relation to the autumn-spawning. Thus, these facts seem to be the relation of evolutionary trend between the spring- summer spawning species and autumn- spawning species.

Intergeneric Hybridization Experiment between Acheilognathus lanceolatus and Rhodeus uyekii

Nobuhiro, Suzuki,Jeon, Sang-Rin 상명대학교 기초과학연구소 1993 기초과학연구 Vol.7 No.-

납자루와 각시붕어의 正을逆交配實驗 實施했던바 각시붕어 암컷과 납자루 수컷 間의 交配에서는 囊胚期까지에 胚發生이 멈췄는데, 납자루 암컷과 각시붕어 수컷 間의 交配에서는 正常的으로 胚發生이 進行되었으며, 孵化仔魚는 約 1 年으로 成熟하여 追星을 나타냈다. 이 交雜種의 仔魚期에는 翼狀突起의 發現을 볼 수 있었으나 이 突起는 각시붕어처럼 發達된 것은 아니었다. 또한 自由遊泳期에 이르는 日齡은 兩親魚보다는 조금 일렀으며, 成魚의 外觀은 兩親魚의 mosic的인 形能였으나 計測, 計數的인 形質에 있어서는 大體로 각시붕어와 近似했다. 交雜種은 모두 수컷으로 間性은 볼 수 없었다. 生殖腺의 光學顯微鏡觀察로는 精小囊의 構造는 正常이였으나 第 1 次 精母細胞의 異常成熟分裂에 依해서 Hematoxylin에 濃染되었는데, 電子顯微鏡觀察에서는 이 細胞는 核에 多量의 heterochromachin을 含有하며 macrophage狀인 細胞에 둘러싸여서 細胞內消化를 받아 消失한다고 推定되었다. 따라서 輸精小管腔에서는 正常的인 精子가 全혀 觀察되지 않았으며 이 때문에 交雜種은 不姙이라고 생각된다. Reciprocal hybrids from the intergeneric crosses between Acheilognathus lanceolatus and Rhodeus uyekii were analyzed morphologically and genetically and then compared with the parental species. The only hybrids bred from female A. lanceolatus were satisfactory to adulthood. These hybrids were generally simillar to R. uyekii in surperficial appearances. All hybrids were sexually males though they were sterile, because of abnormal germ cells occasioned by phagocytosis of spermatocytes.

웅천천(熊川川)수계산(水系産)납자루의 개체발생(個體發生)

영목신양,전상린 한국어류학회 1990 韓國魚類學會誌 Vol.2 No.1

熊川川 水系産 납자루의 個體發生에 對하여 22±1℃의 飼育 水溫下에 있어서 經時的인 觀察을 實施하고 同時에 仔魚의 表皮上突起의 檢討도 實施하여 日本産 납자루와의 比較도 行하였다. 兩國産 납자루는 完熟卵의 形熊가 韓國産이 日本産보다 顯著하게 가느다란 紡錘形인 點으로 區別되었으나 卯發生과 仔魚의 發育形熊 그리고 仔魚의 表皮上突起의 形熊 等에서는 差異를 볼 수 없었으며 初期發生에 있어서 生理的인 隔離가 存在하지 않음이 밝혀졌다. 本 種과 近綠種이라고 생각되는 칼납자루는 日本産과 韓國産 사이에서 個體發生上의 差異나 生理的인 隔離가 있음이 밝혀져 있는데 兩國産 납자루 사이에서는 完熟卵의 形熊만 差異가 있었을 뿐이다. 完熟卵의 形熊에는 亞種 間의 差異나 地理的인 變異가 있음이 알려져 있으므로 兩國産 납자루 사이에는 種 水準의 分化라기 보다는 亞種的 分化 段階에 있다고 생각되었다. The development of eggs and larvae, and minute tubercles on the skin surface of the larvae in Acheilognathus lanceolatus (=Acheilognathus intermedia intermedia) from Korea were observed. The ripe eggs of A. lanceolatus from Korea were nearly spindly. The shape was distinguishable from that of Japan. And then the embryonic development of this specimen from Korea was faster than that from Japan. As regards larval development and morphology and distribution of the minute tubercles, however, A. lanceolatus in both Korea and Japan were same except for the mentioned above two characters. These findings suggested that the two formes should be separated at subspecies level.