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베타카로틴강화미 발현단백질에 대한 항원성연구

박수진(Soo Jin Park),정미혜(Mihye Jeong),장희섭(Hee Seop Chang),오진철(Jin Cheol O),박경훈(Kyung-Hun Park),박재읍(Jae-Yup Park) 한국농약과학회 2011 농약과학회지 Vol.15 No.3
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  ScienceON
  
  DBpia
As part of safely evaluation of 2A (amono acid), PAT (phosphinotricin Acetyl-transferase), CtrI (Carotene desaturase) and PSY (phytoene synthase), the expressed proteins inserted to β-carotene Biofortified rice were tested for antigencity test. As a result, the group of administering high-concentration PAT, the expressed protein, showed a great content of total WBC; however, other expressed proteins did not show much difference. Against ASA (Active Systemic Anaphylaxis) test, the group of administering high-concentration PAT, the expressed protein, showed mild or medium degree of symptoms, but there was no dead entity, According to the result of the PCA (passive Cutaneous Anaphylaxis test), the group of administering high-concentration PAT, 2A, PSY, and mixture of expressed proteins indicated positive response ill low anti-serum concentration, and the group of administering the clinical concentration of mixture indicated mild positive response. However, because the group of administering the clinical concentration of expressed proteins, PAT, 2A, PSY, and CtrI, did not show positive response, it is thought that IgE is not generated. Further studies are needed to verify the safety of β-carotene Biofortified rice.
2
배추좀나방 방제약제로서 후추 추출물, 카시아 오일, 라벤더 오일 함유 친환경유기농자재에 대한 급성 생태독성평가

유아선(Are-Sun You),정미혜(Mihye Jeong),홍순성(Soon-Seong Hong),장희섭(Hee-Seop Chang),이제봉(Je Bong Lee),박경훈(Kyung-Hun Park),이영묵(Young Mook Lee),임양빈(Yangbin Ihm) 한국농약과학회 2013 농약과학회지 Vol.17 No.4
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 ScienceON
 
 DBpia
Environment-friendly agro-materials tend to be preferred to chemical insecticides recently. For this reason, many studies are conducted to develop environment-friendly insecticides containing natural materials. The purpose of this study was to assess ecotoxicity for pepper extract and cassia oil (11.5+46%, A), pepper extract and cassia oil (23+34%, B), and pepper extract and lavender oil (40+10%, C). They are expected to prevent from pests especially diamondback moth, and can be used for agro-materials. Their formulation was emusifiable concentration (EC). Target species used to assess acute toxicity were aquatic invertebrate (Daphina magna), fish (Cyprinus carpio), honeybee (Apis mellifera L.) and earthworm (Eisenia fetida). The EC50 value of A, B, and C to aquatic invertebrate were 0.46, 1.9, 0.25 mg L?1 respectively and these values were moderately toxic according to standard of USEPA. In case of acute toxicity test to fish, the LC50 of A, B, and C were 1.9, 2.9, 3.8 mg L?1 respectively. A was category II in acute toxicity of fish and not acceptable to evaluation criteria of environment-friendly agro-materials. B and C were category III and acceptable. Acute contact and oral toxicity test to honeybee were conducted and the LD50 of A, B, and C were > 100 μg a.i. bee?1 in both of tests. It indicated they were low toxic to honeybee. In case of acute toxicity test to earthworm, LC50 of A, B, and C were 695, 988, and 564 mg kg?1. In conclusion, pepper extract+cassia oil 57% EC and pepper extract+lavender oil 50% EC were expected to be used for environment-friendly insecticide materials with low risk against ecosystem and contribute to developing environment-friendly agro-materials.
3
식물추출물 마늘 추출액, 잔톡실럼 정유, 레몬그라스 정유 함유 유제 3종의 생태독성평가

유아선(Are-Sun You),홍순성(Mihye Jeong),정미혜(Soon-Sung Hong),박경훈(Kyung-Hun Park),장희섭(Hee-Seop Chang),이제봉(Je Bong Lee),박재읍(Jae-Yup Park) 한국농약과학회 2012 농약과학회지 Vol.16 No.4
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 ScienceON
 
 DBpia
Environment-friendly agro-materials are are likely to be preferred to chemical insecticides recently. For this reason, many studies are conducted to develop environment-friendly insecticides containing natural materials. This study was also conducted so as to assess ecotoxicity for Emulsifiable concentrate (EC) containing 30% of garlic extract or two plant essential oils (Zanthoxylum, Lemongrass) expected to prevent from pests and be used for agro-materials. Target species used to assess acute toxicity were invertebrate (Daphina magna), fish (Oryzias latipes), honeybee (Apis mellifera L.) and earthworm (Eisenia fetida). The EC50 values for of garlic extract 30% EC, Zanthoxylum oil 30% EC and lemongrass oil 30% EC to Daphina magna were 3.3, 10, and 10 mg L?1, respectively. The category of garlic extract 30% EC was moderately toxic, while those of Zanthoxylum oil 30% EC and lemongrass oil 30% EC were slightly toxic according to standard of USEPA. EC50 for both of Zanthoxylum oil 30% EC and lemongrass oil 30% EC were more than 10 mg L?1 then they were considered as slightly toxicity. In case of acute toxicity test to fish, LC50 of garlic extract 30% EC was 3.3 mg L?1. Zanthoxylum oil 30% EC and lemongrass oil 30% EC indicated LC50> 10 mg L?1. Classification of acute toxicity to all test substances was in Korea criteria. Acute contact and oral toxicity test to Honeybee were conducted. As a result, LD50 of all test substances were more than 100 a.i. μg bee?1 in the acute contact test while LD50 of garlic extract 30% EC was 4.4 a.i. μg bee?1 and LD50 of Zanthoxylum oil 30% EC and lemongrass oil 30% EC were more than 100 a.i. μg bee?1. In case of acute toxicity test to earthworm, LC50 of garlic extract 30% EC, Zanthoxylum oil 30% EC and lemongrass oil 30% EC were 267, 592, and 430 mg kg?1, respectively. In conclusion, if the safety for earthworm is confirmed, these substances are expected to be use for environment-friendly insecticide materials with low risk against ecosystem and contribute to developing environment-friendly agro-materials.

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