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조직배양에 의한 Cymbidium Kenny Wine Color 식물체 재분화에 미치는 LED 광질 효과
고정애 ( Jeong Ae Ko ),장형목 ( Hyoung Mok Jang ) 전북대학교 농업과학기술연구소 2014 농업생명과학연구 Vol.45 No.2
In order to investigate the effect of the light emitting diode (LEDs) lightings on the induction and multiplication of PLBs (protocorm like bodies) and plant regeneration for the mass propagation, the meristems with one -two leaf primordia of Cymbidium ``Kenny Wine Color`` were cultured on Hyponex media. Fifty days after incubation under various lighting sources of LEDs (red, blue, red+blue, green, and white) as well as fluorescent light, the external characteristics of induced protocorms were determined. The white light was most effective for shoot and root differentiation as well as plant regeneration. Shoot and root length were also highest under white light condition. The fresh weights of protocorms were higher under red light than under fluorescent light condition. Both white and blue lights increased the chlorophyll concentrations of protocorms and leaves compared to fluorescent light. The red light induced the yellowing symptom in protocorm and shoots, resulting in the lowest concentration of chlorophylls in those tissues. Growth characteristics of plants were determined at 300 days after culture. The balanced plantlets were obtained by treating the red+blue mixed light. The growth characteristics of hardened plants were measured at 500 days after culture (200 days after acclimatization). Most of the growth variables were favorable under the red+blue mixed light condition, including leaf number (8.9), leaf length (20.6 cm) and width (7.3 mm), root number (16.0) and length (12.5 cm) and fresh weight (9.2 g), compared to the fluorescent light. The SPAD values were slightly different between red+blue mixed light and fluorescent light. The length of stomata and guard cell on the leaves of hardened plants were significantly larger in fluorescent light (32.3 μm), blue light (32.3 μm), and red+blue light (28.6 μm) than white (21.0 μm) and red lights (22.8 μm).The number of stomata in these leaves were also significantly increased in fluorescent light (6,539/cm2), blue light (5,936/cm2) and red+blue mixed light (7,255/cm2) than white (5,347/cm2) and red lights (5,167/cm2). The greatest number of stomata was counted as 7,552 /cm2 in green light condition.
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