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연구보문 : 환경생물 ; 형질전환 콩 재배가 근권 토양 미생물상에 미치는 영향
이기종 ( Ki Jong Lee ),손수인 ( Soo In Sohn ),이장용 ( Jang Yong Lee ),이부영 ( Bu Young Yi ),오성덕 ( Sung Dug Oh ),권순종 ( Soon Jong Kweon ),서석철 ( Seok Choel Suh ),류태훈 ( Tae Hun Ryu ),김경환 ( Kyung Hwan Kim ),박종석 ( Jo 한국환경농학회 2011 한국환경농학회지 Vol.30 No.4
BACKGROUND: Soybean [Glycine max (L.) Merrill] is legume and an important oil crop worldwide. This study was conducted to evaluate the possible impact of transgenic soybean cultivation on the soil microbial community. METHODS AND RESULTS: Microorganisms were isolated from the rhizosphere soils. Microbial community was identified based on the culture-dependent and molecular biology methods. The total numbers of bacteria, fungi, and actinomycete in the rhizosphere soils cultivated with transgenic and non-transgenic soybeans were similar to each other, and there was no significant difference between transgenic and non-transgenic soybeans. Dominant bacterial phyla in the rhizosphere soils cultivated with transgenic or non-transgenic soybeans were Actinobacteria, Firmicutes, and Proteobacteria. The microbial communities in transgenic and non-transgenic soybean soils were characterized using the denaturing gradient gel electrophoresis (DGGE). The DGGE profiles showed the different patterns, but didn`t show significant difference to each other at 0.05 significance level. DNAs were isolated from soils cultivating transgenic or non-transgenic soybeans and analyzed for persistence of transgenes in the soil by using PCR. PCR analysis revealed that there were no amplified γ-tmt and bar gene in soil DNA. CONCLUSION(S): The results of this study suggested that microbial community of soybean field were not significantly affected by cultivation of the transgenic soybeans.