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유석희 ( Yu Seog Hui ),강응택 ( Kang Eung Taeg ),정상인 ( Jeong Sang In ),양용태 ( Yang Yong Tae ),이병직 ( Lee Byeong Jig ) 대한내과학회 1993 대한내과학회지 Vol.44 No.5
Backgrounds: The pathogenesis of korean hemorrhagic fever (Hemorrhagic fever with renal syndromes) is not elucidated until today. The basic mechaism of disease is vascular injury which increases its permeability. This study took aim to demonstrate the tropism of Hataan virus to human umbilical vein endothelial cells (HUVEC) and the infectivity of virus porliferated in HUVEC. Methods: Cultrued HUVEC were inoculated with Hantaan virus (starin 76-118). Replication of Hantaan virus in HUVEC was demonstrated by immunofluorescence and immunohistochemistry using mouse anti·Hantaan virus monoclonal antibody. The infectivity of replicated virus in was determined with Vero E-6 cells. Results: The infectivity of Hantaan virus was 10⁴. After 3 days after virus inoculation, specific fine granular fluorecsence was found in the cytoplasm of HUVEC. After 7 days nearly all of cells showed specific fluorescence. Infected HUVEC could infect Vero E-6 cells. Conclusions: We demonstrated the tropism of Hantaan virus to cultured HUVEC and infectivity of virus in infected HUVEC.