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Bacillus sonorensis KCTC13918로부터 새로운 laccase유전자 ( soncotA )의 클로닝과 대장균에서의 발현
최신건(Shin-Geon Choi),윤현종(Hyeonjong Yoon) 강원대학교 산업기술연구소 2017 産業技術硏究 Vol.37 No.1
A new putative laccase gene (soncotA) which show 78% homology with that from Bacillus licheniformis (liccotA) was isolated from draft genome sequence of Bacillus sonorensis KCTC 13918. A 1,545 bp of PCR product corresponding 514 amino acids was cloned into NdeI-NotI site of pET21c and expressed as soluble form in E. coli. About 59 kDa size of recombinant laccase was purified into homogenity by Ni-NTA column and laccase activity was confirmed by zymography. The enzymatic properties of recombinant laccase were characterized. The specific activity of B. sonorensis laccase was 0.033 fold lower than that of Bacillus licheniformis laccase. The finding of new laccase gene broadened the enzymatic diversity of Bacillus species laccases.
H형강의 웨브에 콘크리트를 충전한 합성보의 휨 성능 평가
양원직(Yang, Won-Jik),이원호(Yi, Waon-Ho),박진영(Park, Jin-Young),곽한식(Kwak, Han-Sik),윤현종(Yoon, Hyun-Jong),이학수(Lee, Hack-Soo) 대한건축학회 2012 大韓建築學會論文集 : 構造系 Vol.28 No.6
Due to the depression of economy, the resent construction market is in need of new process of the construction technology, which is efficient and economically sustainable development. Especially, due to the increase in steel costs, the research and development for the composite beam of steel frame buildings is actively taking place. Most types of the existing field assembly composite beams are concrete encased composite beams and they can be divided in two types: one is to produce in factories and assembled on site, such as common steel frame structure, the other is to place concrete after making a form around H-Beam. In the former case, the poured concrete increases the weight of beam and it causes a lot of time in handling on site. In the latter case, producing the form in the field results in an increase of construction time. Hence, this study shall develop the composite beam, which is able to solve the problems mentioned above. Besides it shall be verified its performance by experiment.