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이창수(C . S . Lee),유영복(Y . B . Yoo),나기준(K . J . Na),조병대(B . D . Cho),최병규(B . K . Choe) 한국축산학회 1994 한국축산학회지 Vol.36 No.4
Genetic differentiation between Korean Native Cattle (Hanwoo) and European cattle breeds was compared by the analysis of DNA polymorphisms. Genomic DNA of Hanwoo or Holstein breeds was digested using 7 restriction endonucleases. BamHI, BglI. EcoRI, HinfIII, MspI PstI, and TagI. All the enzymes produced clear stained bands. The cleavage patterns of all the enzymes were identical between Hanwoo and Holstein breeds. DNA polymotphisms among cattle breeds were also compared by random amplified polymorphic DNAs (RAPD) analysis based on polymerise chain reaction. Less than 10 polymorphic bands were detected. The RAPD patterns were identical among the Holstein, Hereford and Angus breeds, but the above pattern was different from that of Hanwoo. All bands detected in the Hanwoo breed were observed in European cattle breeds, but one of the common bands found in European cattle breeds was not detected in the Hanwoo breed. Tbis band was independent of sex and individuals, and may be useful as a DNA marker for identifying the Hanwoo breed.