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사료 내 Bacillus 균주의 첨가가 넙치(Paralichthys olivaceus) 치어의 성장, 사료효율, 선천성면역 및 연쇄구균 질병저항성에 미치는 영향
차지훈 ( Ji Hoon Cha ),양시용 ( Si Yong Yang ),우서형 ( Seo Hyung Woo ),송진우 ( Jin Woo Song ),오대한 ( Dae Han Oh ),이경준 ( Kyeong Jun Lee ) 한국수산과학회(구 한국수산학회) 2012 한국수산과학회지 Vol.45 No.1
We investigated the effect of dietary supplementation with probiotics on growth performance, feed utilization, innate immunity and disease resistance against Streptococcus iniae in juvenile olive flounder Paralichthys olivaceus. Four experimental diets were made by supplementation with three Bacillus sp. probiotics (B. subtilis, B. pumilus and B. licheniformis) into a basal diet (control) at 0.5% each. Three-hundred fish (average BW, 20 g) were randomly distributed (25 per tank) into twelve 150 L capacity polyvinyl circular tanks with three replicates per dietary treatment. Triplicate groups of fish were fed the experimental diets to apparent satiation (twice per day, 09:00 and 17:00 h) for nine weeks. After the feeding trial, a challenge test was directly conducted against S. iniae. The results indicated that dietary supplementation with the probiotic, Bacillus sp, seemed to improve growth performance of olive flounder. Hematological parameters and innate immunity as determined by nitroblue tetrazolium, myeloperoxidase or superoxide dismutase activities were not significantly affected by the probiotic supplementation. The result from the challenge test suggested that dietary supplementation with probiotics Bacillus sp. can improve the disease resistance of olive flounder against S. iniae.