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Ti-플라스미드 유전자 운반체에 의한 대두 형질전화 식물체에 있어서 β-Glucuronidase 유전자의 유전양상
김달웅,조은기,서득룡,오웬스, L . D . 경북대학교 유전공학연구소 1995 遺傳工學硏究所報 Vol.10 No.1
To introduce β-glucuronidase (GUS) gene of Escherichia coli into plant cells using an Agrobacterium/binary vector system, we cocultivated shoot apices of soybean with Agrobacterium. After 3 weeks of culture on a culture medium containing 100 ㎎/L kanamycin, kanamycin resistant apices were selected. High GUS activities were detected in transformed explants by spectrophotometric assay. A tumefaciens ET₂[pGT100/EHA101] strain was more effective than the other strains for GUS expression from kanamycin resistant shoots. GUS was expressed in 43% of the cultured explants. It took only 6 weeks to regenerate the rooted plants. When transformants were self-pollinated, GUS gene was inherited to the next generation. Segregation ratio for GUS in T₂ suggests the insertion of a single copy of GUS.