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베타카로틴 강화 GM콩과 야생콩의 교잡종 종자 특성 평가
오성덕(Sung-Dug Oh),장예진(Ye-Jin Jang),박수윤(Soo-Yun Park),서상재(Sang Jae Suh),이범규(Bumkyu Lee) 한국잡초학회·한국잔디학회 2020 Weed & Turfgrass Science Vol.9 No.2
본 연구에서는 야생콩과 베타카로틴 강화콩의 교잡으로 형성된 교잡종의 종자 및 생육 특성과 월동성을 조사하여 교잡종의 잡초화 가능성을 평가하고자 하였다. 야생콩과 베타카로틴 강화 콩을 개화기 일치 등의 인위적으로 교잡한 결과 최종 교잡율은 0.21% 로, 8개체의 교잡종이 선발되었다. 교잡종은 생육 형태는 모본인 야생콩과 유사한 형태를 보였으며, 형성된 협수와 종자 수 및 종자의 크기는 야생콩과 베타카로틴 강화 콩의 중간 형질을 나타냈다. 반면, 종자의 발아 속도는 야생콩보다 크게 증가하여 베타카로틴 강화콩과 유사한 속도를 보였다. 교잡종은 표토조건에서 3개월간 월동한 경우 야생콩과 비슷한 수치의 월동성을 보였으나, 이를 제외한 나머지 조건에서는 야생콩보다 낮은 월동휴면성을 보였다. 베타카로틴 강화콩과 야생콩간의 낮은 교잡율과 월동휴면성을 바탕으로 교잡종이 환경 내 방출되어 생태계 교란을 유발할 가능성은 낮지만, 다양한 요인을 고려하여 장기적인 교잡 후대종의 도입 단백질 활성 평가 및 잡초화 가능성 평가 등을 비롯한 환경 안전성 평가가 필요할 것으로 사료된다. As the cultivation area for genetically modified (GM) crops is increasing continuously, the concerns about the environmental release of GM crops are also growing. Therefore, we conducted this study to verify gene flow from GM soybean, β-carotene enhanced transgenic soybean, to wild-type soybean and analyze the characteristic of hybrid soybean. The hybridization rate between β-carotene enhanced soybean and wild-type soybean was 0.21%. The hybrid soybean showed the highest value on stem length, number of branches and number of nodes than the β-carotene soybean and wild-type soybean. The seeds of hybrid soybean had intermediate characteristics between β-carotene enhanced soybean and wildtype soybean in seed length, seed width, seed weight and seed coat color. The germination time of hybrid soybean was faster than wild-type soybean while the wild-type was similar with β-carotene enhanced soybean. The hybrid soybean showed the lower over-wintering dormancy rates than wild-type soybean after 5 months, and the β-carotene soybean had extremely low or no dormancy rates on all conditions. These results indicate that the hybrid soybean has intermediate characteristics between their parents and will be useful for research in management of GM crops and hybrid crops.
바이러스저항성 삼각주 재배가 토양 미생물상에 미치는 영향
오성덕 ( Sung Dug Oh ),김종범 ( Jong Bum Kim ),이정진 ( Jung Jin Lee ),김민경 ( Min Kyeong Kim ),안병옥 ( Byung Ohg Ahn ),손수인 ( Soo In Sohn ),박종석 ( Jong Sug Park ),류태훈 ( Tae Hun Ryu ),조현석 ( Hyun Suk Cho ),이기종 ( Ki J 한국환경농학회 2013 한국환경농학회지 Vol.32 No.2
BACKGROUND: Genetically modified(GM) trigonal cactus(Hylocereus trigonus Saff.) contained a coat protein gene of cactus virus X (CVX), which conferred resistance to the virus, phosphinothricin acetyltransferase (bar) gene, which conferred herbicide resistance, and a cauliflower mosaic virus 35S promoter (CaMV 35S). This study was conducted to evaluate the possible impact of GM trigonal cactus cultivation on the soil microbial community. METHODS AND RESULTS: Microorganisms were isolated from the rhizosphere of GM and non-GM trigonal cactus cultivation soils. The total numbers of bacteria, and actinomycete in the rhizosphere soils cultivated GM and non-GM trigonal cactus were similar to each other, and there was no significant difference. Dominant bacterial phyla in the rhizosphere soils cultivated with GM and non-GM trigonal cactus were Proteobacteria, Uncultured archaeon, and Uncultured bacterium. The denaturing gradient gel electrophoresis (DGGE) profiles show a similarpatterns, significant difference was not observed in each other. DNA was isolated from soil cultivated GM and non-GM trigonal cactus, we analyzed the persistence of the inserted gene by PCR. Amplification of the inserted genes was not observed in the soil DNA, which was collected after harvest. CONCLUSION(S): This result suggests that the GM trigonal cactus cultivation does not change significantly the microbial community
Protox 제초제저항성 벼 재배가 토양미생물 군집에 미치는 영향
오성덕 ( Sung Dug Oh ),안병옥 ( Byung Ohg Ahn ),김민경 ( Min Kyeong Kim ),손수인 ( Soo In Sohn ),류태훈 ( Tae Hun Ryu ),조현석 ( Hyun Suk Cho ),김창기 ( Chang Gi Kim ),백경환 ( Kyoung Whan Back ),이기종 ( Ki Jong Lee ) 한국환경농학회 2013 한국환경농학회지 Vol.32 No.2
BACKGROUND: Rice (Oryza sativa) is the most important staple food of over half the world`s population. This study was conducted to evaluate the possible impact of transgenic rice cultivation on the soil microbial community. METHODS AND RESULTS: Microorganisms were isolated from the rhizosphere of GM and non-GM rice cultivation soils. Microbial community was identified based on the culture-dependent and molecular biology methods. The total numbers of bacteria, fungi, and actinomycete in the rhizosphere soils cultivated with GM and non-GM rice were similar to each other, and there was no significant difference between GM and non-GM rice. Dominant bacterial phyla in the rhizosphere soils cultivated with GM and non-GM rice were Actinobacteria, Firmicutes, and Proteobacteria. The microbial communities in GM and non-GM rice cultivated soils were characterized using the denaturing gradient gel electrophoresis (DGGE). The DGGE profiles showed similar patterns, but didn`t show significant difference to each other. DNAs were isolated from soils cultivating GM and non-GM rice and analyzed for persistence of inserted gene in the soil by using PCR. The PCR analysis revealed that there were no amplified protoxgene in soil DNA. CONCLUSION(S): These data suggest that transgenic rice does not have a significant impact on soil microbial communities, although continued research may be necessary.
병저항성 GM(OsCK1)벼의 물벼룩(Daphnia magna)에 대한 급성독성 평가
오성덕 ( Sung Dug Oh ),이기종 ( Ki Jong Lee ),박수윤 ( Soo Yun Park ),류태훈 ( Tae Hun Ryu ),서상재 ( Sang Jae Suh ) 한국환경농학회 2014 한국환경농학회지 Vol.33 No.2
BACKGROUND: The disease resistant (OsCK1) rice was generated by inserting choline kinase (CK1) and phosphinothricin acetyltransferase (PAT) genes isolated from Oryza sativa and Streptomyces hygroscopicus into the genome of the rice, Nakdongbyeo. With the potential problems of safeties, the evaluations on non-target organisms are essentially required for the environmental risk assessment of genetically modified (GM) crops. In the present study, we conducted the evaluation of acute toxicity on Daphnia magna that commonly used as a model organism in ecotoxicological studies for non-target organism evaluation. METHODS AND RESULTS: Effect of acute toxicity to Daphnia magna by each concentration were investigated in the disease resistant (OsCK1) rice and non-genetically modified (non-GM) rice, Nakdongbyeo, as concentration (0, 1,000, 1,800, 3,240, 5,830, 10,500 and 20,000 mg/L). The OsCK1 rice used for the test was confirmed to express the OsCK1/PAT gene by the PCR(Polymerase chain reaction) and western blot analysis. Feeding test showed that no significant differences in cumulative immobility and abnormal response of Daphnia magna fed on OsCK1 rice or non-GM rice. The 48hr-EC50 values showed no difference between OsCK1 rice (3,147.18 mg/L) and non-GM rice (3,596.27 mg/L). CONCLUSION: This result suggested that there was no significant difference in toxicity to Daphnia magna between OsCK1 rice and non-GM counterpart.
병저항성 GM(OsCK1)벼가 미꾸리(Misgurnus anguillicaudatus)및 잉어(Cyprinus carpio)에 미치는 영향
오성덕 ( Sung Dug Oh ),이기종 ( Ki Jong Lee ),박수윤 ( Soo Yun Park ),이대용 ( Dae Yong Lee ),손수인 ( Soo In Sohn ),김민영 ( Min Young Kim ),류태훈 ( Tae Hun Ryu ) 한국환경농학회 2013 한국환경농학회지 Vol.32 No.3
BACKGROUND: The disease resistant (OsCK1) rice was generated by inserting choline kinase (CK1) and phosphinothricin acetyltransferase (PAT) genes isolated from Oriza sativa and Streptomyces hygroscopicus into the genome of rice (Nakdongbyeo). With the potential problems of safety, the non-target organism evaluation is required as an essential element for the environmental risk assessment of genetically modified (GM) crops. In present study, we studied the effects on survival of Misgurnus anguillicaudatus and Cyprinus carpio, commonly used as a model organism in ecotoxicological studies. METHODS AND RESULTS: The M. anguillicaudatus and C. carpio were fed on disease resistant (OsCK1) rice and non-genetically modified (non-GM) rice (Nakdongbyeo) to 0, 10, 100, 1,000 and 5,000 mg/L, as treatment concentration respectively. The OsCK1 rice used for the test was confirmed to have the OsCK1/PAT gene expression by the PCR and ELISA analysis. Feeding test showed that no significant differences in cumulative immobility and abnormal response of M. anguillicaudatus and C. carpio fed on between OsCK1 rice and non-GM rice. The 96hr-LC50 values showed no difference between OsCK1 rice (>5,000 mg/L) and non-GM rice (>5,000 mg/L). CONCLUSION(S): The results of this study suggested that there was no significant difference in toxicity for M. anguillicaudatus and C. carpio between OsCK1 rice and non-GM counterparts.
해충저항성 Bt벼의 환경위해성 평가: 해충저항성 Bt벼가 미꾸리(Misgurnus anguillicaudatus) 및 잉어(Cyprinus carpio)에 미치는 영향
오성덕 ( Sung Dug Oh ),이대용 ( Dae Yong Lee ),손수인 ( Soo In Sohn ),이기종 ( Ki Jong Lee ),류태훈 ( Tae Hun Ryu ),이장용 ( Jang Yong Lee ),박범석 ( Beom Seok Park ),권순종 ( Soon Jong Kweon ),서석철 ( Seok Cheol Suh ),박종석 ( J 한국국제농업개발학회 2011 韓國國際農業開發學會誌 Vol.23 No.5
We developed insect-resistant GM rice(Bt transgenic rice) by inserting the mCry1Ac1 a modified gene from the soil bacterium, Bacillus thuringiensis. The Bt transgenic rice expressing the Bttoxin mCry1Ac1 was tested for the effects on survival of Misgurnus anguillicaudatus and Cyprinus carpio, commonly used as a model organism in ecotoxicological studies. M. anguillicaudatus and C. carpio fed 100% ground rice in suspension, using either Bt rice or non-GM counterpart rice(Nakdong). The Bt rice used for the test were confirmed to have the mCry1Ac1 gene expression by the immuno-strip and ELISA analysis. Feeding test showed that no significant differences in cumulative immobility and abnormal response of M. anguillicaudatus and C. carpio fed on between Bt rice and non-GM counterpart rice. The 96hr-LC50 values showed no difference between Bt rice(>1,000mg/L) and non-GM rice(>1,000mg/L). We concluded that there was no significant difference in toxicity for non-target organisms(M. anguillicaudatus and C. carpio) between Bt rice and non-GM counterparts.
오성덕(Sung-Dug Oh),박수윤(Soo-Yun Park),이시명(Si Myung Lee),이기종(Kijong Lee),손수인(Soo-In Sohn),박순기(Soon Ki Park),류태훈(Tae-Hun Ryu) 한국육종학회 2015 한국육종학회지 Vol.47 No.1
The β-carotene biofortified transgenic rice was developed by transforming rice cv. Nakdongbyeo with phytoene synthase (Psy) and carotene desaturase (Crt I) genes isolated from Capsicum and Pantoea. The aim of this study was to perform molecular characterization of rice transformants of T5-T7 generation harboring Psy and Ctr I genes driven by endosperm specific globulin promoter for biosafety evaluation of β-carotene biofortified transgenic rice. The structure and sequence of T-DNA in the transformation vector and the insertion sites, flanking sequences and generational stability of inserted T-DNA in transgenic rice lines were analyzed. The transformation vector consisted of right border, MAR gene, carotenogenic genes unit, herbicide resistance selectable marker unit, MAR gene and left border in sequential order. T-DNA was introduced at the position of 30,363,938-30,363,973 bp of chromosome No. 2 by adaptor-ligation PCR. Stable integration of T-DNA and stable expression of bar gene was confirmed in T5 to T7 generations. It was also confirmed that the backbone DNA of transformation vector containing antibacterial gene was not present in the genome of β-carotene biofortified transgenic rice. HPLC analysis confirmed that carotenoids were consistently detected through T5-T7 generations.
오성덕 ( Sung Dug Oh ),이기종 ( Ki Jong Lee ),박수윤 ( Soo Yun Park ),신혜철 ( Hye Chul Shin ),손수인 ( Soo In Sohn ),박종석 ( Jong Sug Park ),류태훈 ( Tae Hun Ryu ) 한국국제농업개발학회 2014 韓國國際農業開發學會誌 Vol.26 No.1
비타민A 강화벼와 ‘낙동’의 미꾸리(Misgurnus anguillicaudatus)와 잉어(Cyprinus carpio)에 대한 급성독성시험을 실시한 결과 48시간 및 96시간-LC50은 1,000 mg/L이상으로 나타났다. 48시간 및 96시간 무영향농도(NOEC)는 1,000 mg/L이었다. 급성독성 시험기간 중 비타민A 강화벼와 낙동벼간의 pH, DO, 수온, 체중 및 전장에 대한 유의적인 결과는 나타나지 않았다. The carotenoid-biofortified (PAC) rice was developed and confirmed biosynthesis of specific carotenoids (zeaxanthin, a-carotene and β-carotene) in rice endosperm. Also, PAC rice contained herbicide resistance selectable marker by introducing the phosphinothricin acetyltransferase (PAT) gene from the Streptomyces hygroscopicus. For biosafety assessment, the PAC rice expressing the herbicide resistant gene PAT and carotenoid biosynthesis gene PSY/CrtI was tested for the effects on survival of Misgurnus anguillicaudatus and Cyprinus carpio, commonly used as a model organism in ecotoxicological studies. M. anguillicaudatus and C. carpio fed 100% ground rice of suspension, PAC or non-GM counterpart rice (Nakdong). Gene expression of PAC rice was confirmed to have the PAT by the southern blot and western blot analysis. Feeding test showed that no significant differences in cumulative immobility and abnormal response of M. anguillicaudatus and C. carpio fed on between PAC rice and non-GM counterpart rice. The 96hr-LC50 values showed no difference between PAC rice(>1,000mg/L) and non-GM rice(>1,000 mg/L). We concluded that there was no significant difference in toxicity for non-target organisms(M. anguillicaudatus and C. carpio) between PAC rice and non-GM counterparts.
오성덕 ( Sung Dug Oh ),이기종 ( Ki Jong Lee ),손수인 ( Soo In Sohn ),권용정 ( Yong Jung Kwon ),김진서 ( Jin Seo Kim ),이장용 ( Jang Yong Lee ),박범석 ( Beom Seok Park ),권순종 ( Soon Jong Kweon ),서석철 ( Seok Choel Suh ),류태훈· 한국국제농업개발학회 2012 韓國國際農業開發學會誌 Vol.24 No.2
해충저항성 Bacillus thuringiensis (Bt) 벼의 비표적곤충인 벼물바구미(Lissorhoptrus oryzophilus)에 대한 성충 전용살충제 Clothianidin 액상수화제의 살충제 감수성 시험을 실시한 결과, 72시간-LC50은 0.245 ml/L(95% 신뢰한계는 0.195~0.307 ml/L)이었으며, Bt벼의 모본으로 대조로 사용한 낙동벼의 72시간- LC50은 0.257 ml/L(95% 신뢰한계는 0.199~0.331 ml/L)이었다. 72시간-LC50은 낙동벼에서 다소 높았지만, 해충저항성 Bt벼 72시간-LC50이 낙동벼의 95% 신뢰한계 내에 포함되어, 두 품종의 LC50값에 유의성이 없는 것으로 판단된다. Insect-resistant transgenic rice, Bt rice was developed by inserting the mCry1Ac1, a modified gene from the soil bacterium Bacillus thuringiensis (Bt). For the biosafety assessment of this GM rice, we studied the effect on insecticide susceptibility of Rice Water Weevil (Lissorhoptrus oryzophilus) which is a nontarget insect pest and commonly used as a model organism in ecotoxicological studies. The Bt rice was confirmed to have the insertion of T-DNA and the protein expression by the polymerase chain reaction (PCR) and the ELISA analysis, As for the cumulative mortalities of Rice Water Weevil on insecticide, Clothianidin in Bt and non-GM counterpart, Nakdong rices were similar. The 72h-LC50 values of Bt and non-GM rices showed 0.245 ml/L and 0.257 ml/L respectively, As the 72h-LC50 vlaue of Bt rice was included within the 95% confidence limit of that of Nakdong rice slightly higher, the two varieties of the 72h-LC50 values were not significant, Thus, we found out there was a strong possibility that Bt rice didn`t affect the insecticide susceptibilities of nontarget insects.
비타민 A 강화벼 급이가 벼물바구미( Lissorhoptrus oryzophilus)의 살충제 감수성에 미치는 영향
오성덕 ( Sung Dug Oh ),이기종 ( Ki Jong Lee ),박수윤 ( Soo Yun Park ),류태훈 ( Tae Hun Ryu ),김재광 ( Jae Kwang Kim ),손수인 ( Soo In Sohn ),김진서 ( Jin Seo Kim ),하선화 ( Sun Hwa Ha ),박종석 ( Jong Sug Park ),안병옥 ( Byung Ohg 한국환경농학회 2012 한국환경농학회지 Vol.31 No.3
BACKGROUND: The carotenoid-biofortified (PAC) rice was generated by inserting phytoene synthase (Psy) and carotene desaturase (Crtl) genes isolated from Capsicum annuum cv. Nockwang and Pantoea ananatis into the genome of a conventional variety of rice (Nakdongbyeo). In our present study, we studied the effects on insecticide susceptibility of Rice Water Weevil (Lissorhoptrus oryzophilus). METHODS AND RESULTS: The L. oryzophilus were fed on carotenoid-biofortified (PAC) rice and its near non-genetically modified (GM) counterparts (Nakdongbyeo) under 25±1℃, humidity of 60±5%, and photoperiod 16L: 8D for more than 60 days. Ten adults were soaked in the Clothianidin SC solution for 5 second in different concentrations, and were detected the mortalities after 24, 48 and 72 hours respectively. Every experiment was conducted with three replications. The cumulative mortalities of L. oryzophilus exposed to Clothianidin SC were similar between two types of feed administration. CONCLUSION: The results of this study suggested that carotenoid-biofortified rice might not affect the insecticide susceptibilities of Lissorhoptrus oryzophilus.