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양회정 ( Hwoi Jeong Yang ),길기정 ( Ki Jung Kil ),이영종 ( Young Jong Lee ) 대한본초학회 2004 大韓本草學會誌 Vol.19 No.2
N/A Objective : In order to reveal the morphological characteristics of Bupleuri Radix prevailed in Korea, the raw and artificial Bupleurum falcatum L. and the raw B. longiradiatum Turcz. were collected., and their external, internal, and flour states were observed via microscopic examination. Method : The slice of the tested material made by paraffin section technique was colored with Safranine Malachite Green contrast methods, and the flour of it was mounted by the liquid made by the same ratio of each of glycerin, acetic acid, and water, and then observed and photographed by olymphus -BHT. Result 1. The external form of B. falaztum L., plant root, looked like a circular cone and had no room between the joints. However, B. longiradiatum Turcz., plant rhizome, had well-articulated joints where many small roots were attached with a cavity in the middle of pith of its slice. 2. The internal form of B. falcatum L. has cork layers composed of 7~8 lines of cells, and 6~8 secretory cells were found around vitta. The cork layers of B. longiradiatum Turcz. was composed of over 10 lines of cells, and 10~20 secretory cells were distributed around vitta. 3. In the flour form, B. longiradiatum Turcz. contains rhizome epidermal cell, which was not observed in B. falcatum L.. Moreover, stoma was not consistently arranged. Conclusion There were differences in external, internal, and flour states between B. falcatum L. and B. longiradiatum Turcz., so that microscope can be used to distinguish between these two.