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      • KCI등재

        왕자귀나무의 뿌리체편에서 고빈도의 식물체 재분화

        박소영 ( Park So Yeong ),안진권 ( An Jin Gwon ),이위영 ( Lee Wi Yeong ) 한국산림과학회 2003 한국산림과학회지 Vol.92 No.6

        A high frequency plant regeneration system was established using root segments of Albizziu corenna via tissue culture. Cotyledon, hypocotyl, leaf and root explants were cultured on B5 basal medium supplemented either with TDZ(0, 0.04, 0.5, 2.3 and 4.5μM) alone or with TDZ in combination with NAA (2.7 and 5.4μM) or 2,4-D(2.3 and 4.5μM). Shoot primordia were formed when the explants were transferred to B5 medium with 0.04-4.5μM TDZ. Among the explant types tested, only root explants showed high frequency shoot regeneration. Other tissues tested did not respond well to the same culture conditions. Histological observation of the responding explants showed direct development of shoots from endodermal tissues of root segments. The shoots formed could be elongated and further grown by transferring to the medium with 1.44μM GA₃. The shoots rooted either on B5 basal medium or on B5 medium supplemented with 1.44μM GAS. Shoot regeneration potential varied among the explant types. Root explants appeared to possess the highest potentiality for in vitro shoot regeneration.

      • KCI등재

        HPLC를 이용한 한국산 송이의 methyl trans-cinnamate 함량 분석

        이위영 ( Lee Wi Yeong ),박영기 ( Park Yeong Gi ),안진권 ( An Jin Gwon ),가강현 ( Ga Gang Hyeon ) 한국산림과학회 2003 한국산림과학회지 Vol.92 No.5

        The contents of methyl trans-cinnamate, a significant and major aromatic compound in Tricholoma matsutake (pine mushroom), were determined by HPLC (High Performance Liquid Chromatography.). Mushroom sample collected from five sites in Korea were extracted with methanol by ultrasonication. The contents of methyl trans-cinnamate in the pileus and stalk of pine mushroom ranged from 84.8 to 995.7 μg/g and from 4.5 to 45.2 μg/g, respectively. The average contents of methyl trans-cinnamate in the pileus and stalk of pine mushroom from the five sites were 559.1 μg/g and 35.7 μg/g, respectively. From the results of this study, we could conclude that the contents of methly trans-cinnamate varied by different parts (pileus and stalk) and collecting sites. The amounts of methyl trans-cinnamate from tresh T. matsutake were higher than those of dried T. matsutake, Pileus of pine mushroom appeared to contain more methyl trans-cinnamate than did stalk. Additionally, as pileus of pine mushroom developed, the aroma content increased as well.

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