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셀러리와 녹색꽃양배추 중 Flurenoxuron 및 Lufenuron의 안전사용기준 설정
사르다르시드와심 ( Syed Wasim Sardar ),최정윤 ( Jeong-yoon Choi ),변건두 ( Geon-doo Byeon ),조영주 ( Yeong-ju Jo ),아브드엘라지즈술래만아메드이자크 ( Abd Elaziz Ishag ),허장현 ( Jang-hyun Hur ) 한국환경농학회 2021 한국환경농학회 학술대회집 Vol.2021 No.-
Among minor crops, celery and broccoli have been widely cultivated due to their excellent source of vitamins, phenolic compounds, and other nutrients. These crops are susceptible to attack by lepidopterous insects such as (Helicoverpa armigera, Plutella xylostella, and Opsisina arenosella). To control these insects and avoid the illegal use of insecticides, registration of effective insecticides is necessary. Flufenoxuron and lufenuron compounds, are the two promising insecticides, belonging to benzoylphenylurea group. They act as chitin synthesis inhibition in insects, used against a wide range of chewing and sucking insects and phytophagous mites. This study was carried out to establish the pre-harvest interval (PHI) for flufenoxuron and lufenuron in celery and broccoli, respectively. The test compounds flufenoxuron (5% DC) diluted by 1,000 times while acetamiprid+lufenuron mixture (8%+5%) (13% SC) diluted by 2,000 times, and then sprayed two times with 1-week interval at 0, 7, 14, and 21 days before harvest. Both compounds flufenoxuron and lufenuron were extracted by QuEChERs method and analyzed using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). The linearity was excellent (r<sup>2</sup> = ≥0.99) in matrix-matched calibration for both insecticides. The average recoveries at two fortification levels (0.01, and 0.1 mg/kg) were 76%∼100% with the relative standard deviation below 10%. The method limit of quantification (MLOQ) for flufenoxuron and lufenuron were 0.01 mg/kg. The residues of flufenoxuron in celery were 1.66∼3.16 mg/kg while the residues of lufenuron in broccoli were <0.01∼0.24 mg/kg. The maximum residue limit (MRL) set by Korean MFDS for flufenoxuron in celery is 2.0 mg/kg and for lufenuron in broccoli is 0.2 mg/kg. According to the study, flufenoxuron declined to a level below the MRL within 21 days between the last application and harvesting, while the residues of lufenuron in broccoli declined to a level below the MRL within 7 days between the last application and harvesting. Based on our result the Rural Development Administration has already set the PHI of 21 days for flufenoxuron in celery, but PHI for lufenuron in broccoli still needs to be established. Therefore, this study recommends the safe use of acetamiprid+lufenuron mixture 13 % in broccoli as a foliar treatment twice at 7 days before harvest.
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