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돼지에서 Actinobacillus pleuropneumoniae의 혈청학적 진단법에 대한 비교연구
심항섭 ( Hang Sub Shim ),우종태 ( Jong Tae Woo ),조중현 ( Jung Huin Cho ),전무형 ( Moo Hyung Jun ) 한국동물위생학회 1994 한국동물위생학회지 (KOJVS) Vol.17 No.2
To establish an effective diagnostic measure for detection of the antibodies against Actinobacillus pleuropneumoniae, the methods for tube agglutination test(TAT), plate agglutination test(PAT), micro-agglutination test(MAT) and agar-gel immunodiffusion test(ID) were improved and standarized, and the comparative studies were carried out. The results obtained through the experiments were summarized as follows. 1. The rabbit hyperimmune sera to reference serotypes 1 to 6 were cross-tested with TAT, PAT, MAT and ID. In the homologous systems, the range of antibody titers in TAT was 80 to 640, showing the cross-reaction in serotypes 3, 4, 5 and 6. The range of antibody titers in PAT was 4 to 64, showing the cross-reaction in serotypes 3, 4, 5 and 6. In ID, the range of antigen titers was 8 to 32, and cross-reaction was observed in serotype 5. 2. The optimal concentration of antigen in PAT and MAT were 100mg/ml and l.25mg /ml respectively. The most sensitive reaction in MAT was observed in 52°C for 18hrs. 3. In ID, the most promising antigen and the buffer for agar-gel were EDTA-treated antigen and 0.05M tris buffer(pH 7.2), respectively. 4. By the tests for 200 swine sera, it was found that the frequency of positive reaction were 203 in TAT, 240 in PAT and 163 in ID. 5. When compared the titers of TAT with those of MAT for 200 swine sera, MAT showed the higher titer than TAT being increased by relative correlation. Int was found that the titer for positive readings were 20 in TAT and 40 in MAT. 6. when compared the results of ID with those of TAT for 200 swine sera, all sera with TAT titer under 10 were negative in ID. Of the sera with TAT titer 20 and 40, 55.1% nd 91.8% were positive in ID, respectively. All sera with TAT titer above 80 were positive in ID. In comparison of ID and MAT, all sera with MAT titer under 20 were negative in ID. Of the sera with MAT titer 40 and 80, 24.7% and 93.9% were positive in ID, respectively. All sera with MAT titer over 160 showed positive in ID. 7. In conclusion, the established MAT showed high sensitivity but low specificity, wherease ID revealed low sensitivity but high specificity.
Latex 응집반응 및 polymerase chain reaction을 이용한 돼지톡소플라즈마병 감염실태 조사
심항섭 ( Hang Sub Shim ),최경묵 ( Gyeong Muk Choi ),전오숙 ( Oh Sook Jeon ),이수진 ( Su Jin Lee ),우종태 ( Jong Tae Woo ),노기완 ( Ki Woan Ro ) 한국동물위생학회 2008 한국동물위생학회지 (KOJVS) Vol.31 No.1
Between March and October 2007, a total of 516 blood samples from pigs in the Gyeonggi province were examined for seroprevalence against toxoplasmosis by latex agglutination test (LAT) and the detection of antigenic particles among seropositive samples by PCR. In the LAT, 118 (22.8%) were positive, and the unadjusted percentage of seroprevalence rates of breeding and fattening pigs were significant difference. Positive rate (14.1%) in the breeding pigs was much lower than that (27.8%) of the fattening pigs (p<0.001, Pearson`s Chi-square test). The antibody detection rate of sows was lower than fattening pigs, i.e., 15.8% (25/158) and 26% (93/358), respectively (P=0.011, Pearson`s Chi-square test). Among 118 seropositive samples by LAT, 68 (57.6%) were positive in PCR for the detection of the toxoplasma specific-DNA. There was a statistical difference in the positive PCR reaction between the raising pigs(63/93 67.7%) and sows (5/25, 20%) (P<0.01).
Enzyme-Linked Immunosorbent Assay(ELISA)를 이용한 혈청 및 원유 중의 Mycobacterium bovis 항체 검출에 관한 연구
심항섭 ( Hang Sub Shim ),국정희 ( Jung Hee Kook ),박병옥 ( Byoung Ok Park ),김성열 ( Sung Youl Kim ),박유순 ( Yu Soon Park ) 한국동물위생학회 1997 한국동물위생학회지 (KOJVS) Vol.20 No.2
In order to supplement a diagnostic method for detection of infectious cattle to bovine tuberculosis, performed ELISA for detection of antibody to M bovis in serum and milk. The diagnostic efficacy of the established ELISA was compared with test of the tuberculin skin test for bovine tuberculosis. The positive corresponding rate of serum ELISA and tuberculin skin test showed 84.3%, milk ELISA and tuberculin skin test showed 75.0%, milk ELISA and serum ELISA showed 75.0% respectively. Comparison of the serum and milk to tuberculin antibody concentration in tuberculin positive cattle, the milk contained 1/100-1/150 concentration compared serum tuberculin concentration. The established ELISA was considered efficient for detection of antibodies to M bovis in serum and milk.
효소면역법을 이용한 Brucella abortus 항체 검출에 관한 연구
심항섭 ( Hang Sub Shim ),국정희 ( Jung Hee Kook ),정봉수 ( Bong Su Chung ),고태오 ( Tae Oh Ko ),조중현 ( Jung Hyun Cho ),박유순 ( Yu Soon Park ) 한국동물위생학회 1998 한국동물위생학회지 (KOJVS) Vol.21 No.2
In order to establish a rapid, sensitive and specific diagnostic method for detection of antibody to Brucella abortus, a enzyme-linked immunosorbent assay(ELISA) was adapted. The diagnostic efficacy of the established ELISA was compared with that of the standard tube agglutination test for B abortus. 1. It was found that the optimal concentration of antigen for this ELISA was 51ug/m1, the optimal dilution of conjugate was 1:2000, and the optimal dilution of serum was 1:200, respectively. 2. Cut off value in this ELISA was 1,102 that was determined by mean absorbance(at 492nm) of tube agglutination test negative serum added with the triple value of the standared devation. 3. The relationship between the tube agglutination test and ELISA was showen high corresponding rate with sensitivity(96.3%) and specificity(98.1%). 4. The efficacy of the ELISA for detection of B abortus antibody was compared with tube agglutination test in brucellosis outbreak farm. The sensivity of ELSIA was higher than tube agglutination test.
심항섭 ( Hang Sub Shim ),국정희 ( Jung Hee Kook ),황영옥 ( Young Ok Hwang ),정봉수 ( Bong Su Jung ),김학열 ( Hak Yeol Kim ),이모란 ( Mo Ran Lee ),유성종 ( Sung Jong Yoo ),강순근 ( Sun Kun Kang ),임경애 ( Kyung Ae Lim ),고태오 ( Ta 한국동물위생학회 1998 한국동물위생학회지 (KOJVS) Vol.21 No.3
Since bovine leukosis caused considerable economic loss to the dairy industry, seroepidemiological survey on bovine leukosis was carried out for the dairy herds in Kyunggi province. 1. When compared the results of immunodifussion test with those of enzyme-linked immunosor-bent assay(ELISA) for 94 dairy herds sera, the relationship between the immunodifussion test and ELISA were showen high corresponding rate with sensitivity(97.5%) and specificity(92.6%). 2. In immunodiffusion test for bovine leukosis virus (BLV) antibody in 570 dairy cattle from 30 herds, mean positive rate for BLV antibody was 28.2 %. The positive rate by districts were 16.5% in central, 35.4% in east, 17.3% in west, 29.1% in south, 31.6% in north, 43.7% in northeast. 3. When the results of serological studies was analyzed by age groups, the number of positive was increased gradually with the advanced in age of herds. The highest positive rate was found in the age over 6 years. 4. Of 30 dairy herds examined, 5 herds(16.7%) have no reactions against BLV antigen while 15 herds (50%) showed the range of 1~5 positive cattle and 5 herds(16.7%), the rang of over 11 positive cattle.
경기도에서 발생하는 유우부루셀라병에 관한 연구: 1. 감염우의 역학조사 및 분리균의 특성에 관하여
심항섭 ( Hang Sub Shim ),고태오 ( Tae Oh Ko ),유성종 ( Sung Jong Yoo ),우종태 ( Jong Tae Woo ),박병옥 ( Byoung Ok Park ),김성열 ( Soung Yeol Kim ),박유순 ( Yoo Soon Park ) 한국동물위생학회 1996 한국동물위생학회지 (KOJVS) Vol.19 No.3
The survey was performed to provied details about the pattern of bovine brucellosis occurred in Kyunggi province area. The results obtained through the investigations were summarized as follows. Five hundred seventy-three cattle of bovine brucella reactor were occurred in 14 districts among 31 districts in Kyunggi province in 1989-1995. Among them, 370 cattle(64.5 %) were bred in Eastern area(Ichon, Yeju, Kwangju) and 153 cattle(26.7%) in Southern area(Yongin, Ansung, Peungtack). And the number of farms occurred by bovine brucellosis was 110 ones. When we investigated the occurrance frequency for the 110 farms, the ratio of farms which was brocken out just one time was 67.3% and more than twice was 32.7%. In 573 cattle, 271 cattle were reoccurred in farms which had broken out the bovine brucellosis more than one time. And this survey said the interval of reoccurrance was like this; within a month 50.2%, within two month 19.2%, within four month 7.4%, within six month 7.4%, within an year 15.1%, within 2 year 7.0%. Brucella abortus was isolated from 38 cattle of the 61 ones cattles, and in type all isolated belong to biotype 1.
단보 : 경기도 북부지역 젖소 사육농장의 bovine leukemia virus 감염 실태 조사
정광 ( Kwang Jung ),심항섭 ( Hang Sub Shim ),백진주 ( Jin Joo Baek ) 한국동물위생학회 2012 한국동물위생학회지 (KOJVS) Vol.35 No.4
This study was carried out to investigate the prevalence of bovine leukemia virus (BLV) infection and to compare the results of enzyme-linked immunosorbent assay (ELISA) and nested polymerase chain reaction (nPCR) in dairy farms in northern Gyeonggi province from August through December 2011. A total of 625 dairy cattle from 14 dairy farms were tested for antibodies against BLV using commer-cially available ELISA test kit. The overall seroprevalence of BLV infection was 76.3%. The seropreva-lence of diary cattle according to age was the highest at 61∼72 months (88.0%, P<0.001). Two hun-dred fifty one dairy cattle from 7 diary farms were tested ELISA and nPCR. The kappa value of BLV between ELISA and nPCR was 0.765. The results indicate that BLV infection spread widely in dairy farms and the nPCR is rapid method for the early detection of BLV infection.
양병훈 ( Byung-hun Yang ),심항섭 ( Hang-sub Shim ) 한국동물위생학회 2016 한국동물위생학회지 (KOJVS) Vol.39 No.1
Protozoan parasite, the genus Eimeria, causes an intestinal disease, coccidiosis, in young broilers. Coccidiosis induces significant economic loss in poultry production. This study was designed to identify the prevalence of Eimeria spp. in broilers in Gyeonggi province, Korea. Fecal samples from broilers at 94 farms were collected in two chicken slaughterhouses between March and June 2015. Eimeria infection was determined by microscopic examination and PCR using ITS-1 region. The prevalence of Eimeria was 58.5% (55 of 94). E. acervulina was identified in 96.4% of samples, E. tenella in 81.8%, E. maxima in 12.7%, E. praecox in 12.7%, E. brunetti in 5.5%, E. necatrix in 1.8%, and E. mitis in 1.8%. Body weight (BW) of broilers infected with both E. tenella and E. acervulina (mean=1.53±0.19B kg) was significantly lower than broilers with E. acervulina positive/E. tenalla negative (mean=1.74±0.07A kg) or Eimeria negative (mean=1.65±0.15A kg) (Duncan’s multiple range test, P<0.01). High prevalenceof Eimeria tenella and the impact on the broiler body weight shows the importance of this protozoa in broiler industry. Development of the periodic monitoring strategy and systemic management for the purpose of the prevention/eradication of Eimeria infection among broilers is required.
김정태 ( Jeong Tae Kim ),심항섭 ( Hang Sub Shim ),김태종 ( Tae Jong Kim ),고태오 ( Tae Oh Ko ),우종태 ( Jong Tae Woo ),유기승 ( Ki Syng Ryu ),박유순 ( Yu Soon Park ) 한국동물위생학회 1998 한국동물위생학회지 (KOJVS) Vol.21 No.3
In order to establish a sensitive and specific diagnostic method for detection of antibody to Salmonella pullorum, a enzyme-linked immunosorbent assay(ELISA) was designed and standardized. The diagnostic efficacy of the established ELISA was compared with that of the serum plate agglutination test and immunodiffusion test for pullorum disease. 1. The chicken hyperimmune sera to Salmonella pullorum, S gallinarum, S typhimurium and S typhi were shown the cross reaction to S pullorum antigen by serum plate agglutination test. 2. When compared the cross reaction titer of microplate agglutination test for chickens hyperim-mune sera, it was found that the titer were 64 in S pullorum, 32 in S gallinarum, 4 in S typhimurium and 8 in S typhi, respectively. 3. When compared the specificity of various antigen(HA, EA, PA and SA) by the immunodiffusion test, the most suitable antigen was phenol-treated bactrium. 4. The optimal concentration of S pullorum antigen for ELISA was 1:160 dilution of bacterium. 5. The efficacy of the ELISA for detection of S pullorum antibody was compared with serum plate agglutination test and immunodiffusion test in chickens infected with S pullorum. The antibody was first detected at 6 days after infection using three tests examined. The antibody was alldetected at 9 days by ELISA, at 12 days by serumplate agglutination test, at 15 days by immuno-diffusion test.
경기지역의 우결핵 발생에 관한 역학적 고찰(1987~1996)
우종태 ( Jong Tae Woo ),심항섭 ( Hang Sub Shim ),고태오 ( Tae Oh Ko ),국정희 ( Jung Hee Kook ),박유순 ( Yu Soon Park ),조중현 ( Jung Hyun Chi ) 한국동물위생학회 1998 한국동물위생학회지 (KOJVS) Vol.21 No.1
As the result of epidemiological analysis on bovine tuberculosis in Kyonggi province during the last ten years(1987-1996), 1. The annual incidence number of bovine tuberculosis positive farms in Kyonggi province fell down from 81 in 1987 to 14 in 1989. But the incidence is increased since 1990, in 1995 the incidence number was 102 farms of the most incidence. Total number of bovine tuberculosis positive farms were 456 farms(56.7%) in Kyonggi province during the 1987-1996 period. 2. The developmental trends of bovine tuberculosis positive heads was similiar to that of positive farms. Since 1993, the incidence was suddenly increased. Total number of bovine tuberculosis positive heads were 1,015 head(64.3%) in Kyonggi province during the 10 years. 3. Average incidence for rate during the last ten years(1987-1996) was 0.10%. The positive rate was suddenly increased since 1993 and in 1996, the positive rate was 0.23% of the highest incidence. 4. Regional incidence number of bovine tuberculosis positive heads in Kyonggi province during the last seven years(1990-1996) was the most in Hwasung, Pochon, Pyungtek in order of incidence and bovine tuberculosis positive density was 1.297% of the highest in Buchon. 5. In 244 farms(82.4%), bovine tuberculosis first occurred during the last seven years (1990-1996) but in farms over second occurrence, the number of bovine tuberculosis positive heads were 520 heads(58.2%).