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FISH법을 이용한 낙동강 상,중,하류의 세균군집 구조 분석
이영옥 ( Lee Yeong Og ),박지은 ( Park Ji Eun ),신승필 ( Sin Seung Pil ),안영희 ( An Yeong Hui ) 한국물환경학회 2003 한국물환경학회지 Vol.19 No.3
For conducting the comparative analysis of the eubacterial community structure including nitrifying bacteria at 8 sampling sites throughout the N마-Dong River, FISH(fluorescence in situ hybridization) method was employed. The total ratio of each determined eubacterial group such as α·β·γ-subclasses protebacteria and Cytophaga-Flavobacterium(CF) group to total counts stained by DAPI at each site varied 9.3∼42.5% with the highest value at uppermost part. And each ratio of determined eubacterial groups reached mostly under 10% except those of CF group(23%) at uppermost part. Futhermore, compared to lower part, upper part represented higher proportions of γ-subclass proteobacteria comprised in most of fast growing bacteria on degradable organics. Also the variations of ammonia-oxidizing bacteria ranged from 2.7×10⁴to 18.3×10⁴ cells/㎖ with the lowest value in lower part and the highest value in mid part whereas those of nitrite-oxidizing bacteria varied 5.2∼7.7×10⁴cell/㎖ without noticeable differences throughout the sites. Additionally, the ratio of nitrifying bacteria to total counts ranged from 1.3% to 13.6?%, regardless of those of ammonia/nitrite-oxidizing bacteria. Overall, it implies that the upper part of Nak-Dong River represented comparatively better degradabilities of organic materials than their Lower part except at one site in upper part passed An-Dong City. By the contrast, the other part of Nak-Dong River, especially after merging the Gum-Ho River revealed lower detectability of FISH resulted from their cellular activity. So it could be said that the lower part of Nak-Dong River might be a ecosystem of which physico-chemical environment may have inhibited the activities of bacteria functioned as decomposer of organic matter.
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