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- 저자 : 신도빈 ( D B Shin ) (검색결과 2 건)
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맛 버섯 자실 체의 메탄올 및 열수추출물의 항산화 및 항염증 활성
( Trung Kien Nguyen ),신도빈 ( Do Bin Shin ),이수민 ( Su Min Lee ),임경환 ( Kyung Hoan Im ),이태수 ( Tae Soo Lee ),이우윤 ( U Youn Lee ) 한국균학회 2013 韓國菌學會誌 Vol.41 No.2
Pholiota nameko is an edible mushroom belonged to Family Strophariaceae of Agaricales, Basidiomycota. The purpose of this study was to investigate the antioxidant and anti-inflammatory activities for the methanol and hot water extracts prepared from fruiting bodies of Pholiota nameko. Besides measuring for 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity, a reducing power and a chelating activity on ferrous ions were also measured to evaluate the antioxidant activity for those extracts. To measure the anti-inflammatory activities for the extracts, nitric oxide(NO) production from lipopolysaccharide treated RAW 264.7 macrophage cells and carrageenan-induced acute hind paw edema of rats were investigated. The results showed that those extracts has a excellent chelating activity on the ferrous ions compared with positive controls. And it also turned out that extracts had a good DPPH activity and a reducing power. The NO production in LPS-treated RAW 264.7 macrophage cells were decreasing as we increased concentration of those mushroom extracts. Significant reduction of paw edema were also observed at 2~6 h after we treated methanol and hot-water extracts at the 50 mg/kg concentration to the rats which are induced acute hind paw edema by carrageenan treatment. The experimental results suggested that methanol and hot-water extracts of Pholiota nameko fruiting bodies might be used for potential source of antioxidant and anti-inflammatory agents.
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이재성 ( J S Lee ),신도빈 ( D B Shin ),이수민 ( S M Lee ),김성환 ( S H Kim ),이태수 ( T S Lee ),정덕채 ( D C Jung ) 한국균학회 2013 韓國菌學會誌 Vol.41 No.2
Phellinus baumii is a medicinal mushroom used in Asian countries for a long period of time. The purpose of this study was to investigate the skin whitening activities of methanol extracts from fruiting bodies of P. baumii. To evaluate the antioxidant activities of the extract, polyphenol and flavonoid contents, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity and chelating activity on ferrous ions were studied. For assay of skin whitening activities, tyrosinase and DOPA inhibitory activities, and tyrosinase and melanin synthesis inhibitory activities of B16/F10 melanoma cells treated with the methanol extract were investigated. The total polyphenol content of P. baumii methanol extract was 4.19. DPPH scavenging ability of P. baumii methanol extract was 88.26% in 25 μg/mL concentration. We tested tyrosinase inhibitory activity and melanin contents in melanoma cells. The tyrosinase activity was inhibited to 65.17% at the concentration of 125 μg/mL and melanin synthesis was inhibited to 57.61% at the concentration of 25 μg/mL. Overall, the experimental results showed that P. baumii methanol extract had inhibitory activities of tyrosinase and melanin synthesis by dose dependent manner in B16/F10 melanoma cells. Strong ultra-violet absorption spectra in the range of 270~370 nm indicated that ethanol extract of P. baumii could protect the skin from UV. Therefore, P. baumii methanol extract might be used for development of skin whitening, anti-UV and skin care agents.
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