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마이크로플레이트 기반 생물반응기 시스템 (MABOOMS™)을 이용한 대장균 배양공정에서 용존산소, pH 및 세포농도의 온라인 모니터링 연구
손옥재(Ok-Jae Sohn),이종일(Jong Il Rhee) 한국생물공학회 2013 KSBB Journal Vol.28 No.1
Dissolved oxygen, pH and cell concentration have been online monitored in cultivation processes with Escherichia coli by using a MABOOMS™ (microplate-based bioreactor with optical online monitoring systems). Fluorescent sensing membranes containing Ru (dpp)₃<SUP> 2+</SUP> or HPTS were prepared with GA sol-gel matrix and coated into a well of a 24-well microplate. Fluorescence intensity was measured and correlated to the dissolved oxygen or pH. Cell concentrations were also online monitored by measuring optical reflectance at 650 nm. A well of a 24-well microplate could also be divided into 4 parts, each of which was coated with fluorescent sensing membranes for the detection of dissolved oxygen or pH. The 24-well microplate coated with fluorescent sensing membranes or a 4-divided sensing membrane. was used to online monitor the dissolved oxygen, pH and cell concentration during E. coli cultivations. The online monitoring results showed the characteristics of cell growth in cultivation processes very well.
마이크로플레이트 기반 생물반응기 시스템(MABOOMS™)을 이용한 발효배지 성분의 미생물 성장 특성 연구
손옥재(Ok-Jae Sohn),이종일(Jong Il Rhee) 한국생물공학회 2013 KSBB Journal Vol.28 No.1
In this work a MABOOMS™ has been employed to cultivate microorganisms and investigated the effects of culture medium components on cell growth. A 24-well microplate coated with 4-divided fluorescent sensing membranes was used to monitor the dissolved oxygen, pH and cell concentration during cultivations. Fluorescence intensity for dissolved oxygen or solution pH and reflectance for cell concentration was online monitored by using the MABOOMS™. The online monitoring results showed the effects of culture medium components on cell growth in cultivation processes very well.
생물공정 모니터링을 위한 광섬유 포도당 및 젖산 센서의 개발
정창환(Chang Hwan Jung),손옥재(Ok-Jae Sohn),이종일(Jong Il Rhee) 한국생물공학회 2017 KSBB Journal Vol.32 No.1
In this work the optical fiber glucose and lactate biosensors were developed by using fluorescent dye and enzyme immobilized on the end tip of an optical fiber. 3-Glycidyloxypropyl) methyldiethoxysilane (GPTMS), (3-Aminopropyl)trimethoxysilane (APTMS) and Methyltrimethoxysilane (MTMS) were used to immobilize glucose oxidase (GOD), lactate oxidase (LOD) and ruthenium(II) complex (tris(4,7-diphenyl-1,10-phenanthroline) ruthenium(II), Ru(dpp)3<SUP>2+</SUP>) as oxygen sensitive fluorescent dye. MTMS sol-gel was an excellent supporting material for the immobilization of Ru(dpp)3<SUP>2+</SUP>, GOD, and LOD on the optical fiber. Storage stability of the optical fiber glucose sensor was kept constant over 20 days, while the optical fiber lactate sensor had constant storage stability over 17 days. The optical fiber glucose and lactate biosensors also maintained good operational stability for 20 hours and 14 hours, respectively. The activities of the immobilized enzymes were most excellent at pH 7 and at 25℃. On-line monitoring of glucose and lactate in a simulated process was performed with the optical fiber glucose and lactate biosensors. On-line monitoring results were agreed with those of off-line data measured with high performance liquid chromatography (HPLC).