연구논문 : 안전한 화장품 개발을 위한 동물실험대체법 연구 동향

서병휘 ( Byung Fhy Suh ),문성준 ( Seong Joon Moon ),안수선 ( Su Sun An ),김서영 ( Seo Young Kim ),장이섭 ( Ih Seop Chang ) 한국동물실험대체법학회 2007 동물실험대체법학회지 Vol.1 No.1

The AMOREPACIFIC Corp., a leading cosmetic company in South Korea, has been making an effort to use variety of alternative methods for many years. We have been making use of alternative methods on assessing phototoxicity of fragrances, corrosivity of hair care products, and ocular irritancy of eye creams and eye makeup products. In addition to existing protocols, there are also several trials on improving and applying current alternative methods. We have intended to apply the HET-CAM (Hen`s egg test on the chorioallantoic membrane) test to various studies on safety assessment of cosmetic products prior to ophthalmology test or anti-irritant material screening. A battery test was introduced for the purpose of improving the accuracy of in vitro phototoxicity method. We also make effort to get suitable alternative methods for predicting sensitizing potency of cosmetic ingredients and products. The one of the strong candidates is h-CLAT (human cell line activation study) and which is now under examination of its accuracy and utility.

안전한 화장품 개발을 위한 동물실험대체법 연구 동향

서병휘 ( Byung Fhy Suh ),문성준 ( Seong Joon Moon ),안수선 ( Su Sun An ),김서영 ( Seo Young Kim ),장이섭 ( Ih Seop Chang ) 한국동물실험대체법학회 2007 동물실험대체법학회지 Vol.1 No.1

The AMOREPACIFIC Corp., a leading cosmetic company in South Korea, has been making an effort to use variety of alternative methods for many years. We have been making use of alternative methods on assessing phototoxicity of fragrances, corrosivity of hair care products, and ocular irritancy of eye creams and eye makeup products. In addition to existing protocols, there are also several trials on improving and applying current alternative methods. We have intended to apply the HET-CAM (Hen`s egg test on the chorioallantoic membrane) test to various studies on safety assessment of cosmetic products prior to ophthalmology test or anti-irritant material screening. A battery test was introduced for the purpose of improving the accuracy of in vitro phototoxicity method. We also make effort to get suitable alternative methods for predicting sensitizing potency of cosmetic ingredients and products. The one of the strong candidates is h-CLAT (human cell line activation study) and which is now under examination of its accuracy and utility.

연구논문 : 안점막자극시험의 대체시험법으로서의 CAM-TBS assay 소개

김채욱 ( Chae Wook Kim ),정경미 ( Kyoung Mi Jung ),서병휘 ( Byung Fhy Suh ),김배환 ( Bae Hwan Kim ) 한국동물실험대체법학회 2007 동물실험대체법학회지 Vol.1 No.2

The hen`s egg test-chorioallantoic membrane (HET-CAM) assay, which employs the vascular membrane of a fertile hen`s egg, has been actively studied by several countries as an alternative method to the Draize rabbit eye irritation test. However, the scoring system of the original HET-CAM test is not satisfactory because of its lack of objectivity and quantitativeness. Moreover, the test required skill when the effects of test substance are to be regarded. To overcome these disadvantages, Hagino et al. developed and objective evaluation technique using the amount of trypan blue absorbed at the site of treatment as an indicator of injury to the CAM (Chorioallantoic membrane-trypan blue staining, CAM-TBS). In this study, we evaluate the CAM-TBS methods and used three materials (1%, 2%, 5% SLS, tween 20 and PBS) to evaluate the correlation of CAMTBS with HET-CAM test. The amounts of trypan blue adsorbed with the CAM showed a good correlation with the HET-CAM test score(r=0.958). According to these results, we suppose that the trypan blue staining assay using CAM-TBS may be a good method to replace the HET-CAM test as an alternative to Draize rabbit eye irritation test. Despite our results, additional studies with more materials are necessary to validate this method.

안점막자극시험의 대체시험법으로서의 CAM-TBS assay 소개

김채욱 ( Chae Wook Kim ),정경미 ( Kyoung Mi Jung ),서병휘 ( Byung Fhy Suh ),김배환 ( Bae Hwan Kim ) 한국동물실험대체법학회 2007 동물실험대체법학회지 Vol.1 No.2

The hen`s egg test-chorioallantoic membrane (HET-CAM) assay, which employs the vascular membrane of a fertile hen`s egg, has been actively studied by several countries as an alternative method to the Draize rabbit eye irritation test. However, the scoring system of the original HET-CAM test is not satisfactory because of its lack of objectivity and quantitativeness. Moreover, the test required skill when the effects of test substance are to be regarded. To overcome these disadvantages, Hagino et al. developed and objective evaluation technique using the amount of trypan blue absorbed at the site of treatment as an indicator of injury to the CAM (Chorioallantoic membrane-trypan blue staining, CAM-TBS). In this study, we evaluate the CAM-TBS methods and used three materials (1%, 2%, 5% SLS, tween 20 and PBS) to evaluate the correlation of CAMTBS with HET-CAM test. The amounts of trypan blue adsorbed with the CAM showed a good correlation with the HET-CAM test score(r=0.958). According to these results, we suppose that the trypan blue staining assay using CAM-TBS may be a good method to replace the HET-CAM test as an alternative to Draize rabbit eye irritation test. Despite our results, additional studies with more materials are necessary to validate this method.

연구논문 : 토끼 안점막자극 대체시험법으로서의 HET-CAM assay 소개

김채욱 ( Chae Wook Kim ),정경미 ( Kyoung Mi Jung ),이지해 ( Ji Hae Lee ),윤준원 ( Jun Won Yun ),서병휘 ( Byung Fhy Suh ),김배환 ( Bae Hwan Kim ) 한국동물실험대체법학회 2007 동물실험대체법학회지 Vol.1 No.1

The hen`s egg test-chorioallantoic membrane(HET-CAM) assay, which employs the vascular membrane of a fertile hen`s egg, has been actively studied by several countries as an alternative method to the Draize rabbit eye irritation test. There are two methods to evaluate the eye irritation potential, reaction time method(RTM) and end point assessment(EPA), between which can be chosen as the apt method depending on the characteristics of test material. In this study, we employed the EPA method to compare with the Draize rabbit eye irritation test method and then calculated the Pearson correlation coefficient value. The Result of Pearson correlation was high(0.986, p=0.002) with 5 test materials: 3 raw materials(IPMS 03, VCP-IS-2Na, Seletinoid: Amorepacific R&D center, Korea), 1 commercial cleansing form(Amorepacific R&D center, Korea) and 1 surfactant (Amisoft LS-11, Ajinomoto, Japan). According to these results, we suppose that the HET-CAM assay is a good method to replace the Draize eye irritation test as the tests of 5 materials have proven. Despite our results, additional studies with more materials are necessary to validate this method as an alternative to the Draize rabbit eye irritation test method.

토끼 안점막자극 대체시험법으로서의 HET-CAM assay 소개

김채욱 ( Chae Wook Kim ),정경미 ( Kyoung Mi Jung ),이지해 ( Ji Hae Lee ),윤준원 ( Jun Won Yun ),서병휘 ( Byung Fhy Suh ),김배환 ( Bae Hwan Kim ) 한국동물실험대체법학회 2007 동물실험대체법학회지 Vol.1 No.1

The hen`s egg test-chorioallantoic membrane(HET-CAM) assay, which employs the vascular membrane of a fertile hen`s egg, has been actively studied by several countries as an alternative method to the Draize rabbit eye irritation test. There are two methods to evaluate the eye irritation potential, reaction time method(RTM) and end point assessment(EPA), between which can be chosen as the apt method depending on the characteristics of test material. In this study, we employed the EPA method to compare with the Draize rabbit eye irritation test method and then calculated the Pearson correlation coefficient value. The Result of Pearson correlation was high(0.986, p=0.002) with 5 test materials: 3 raw materials(IPMS 03, VCP-IS-2Na, Seletinoid: Amorepacific R&D center, Korea), 1 commercial cleansing form(Amorepacific R&D center, Korea) and 1 surfactant (Amisoft LS-11, Ajinomoto, Japan). According to these results, we suppose that the HET-CAM assay is a good method to replace the Draize eye irritation test as the tests of 5 materials have proven. Despite our results, additional studies with more materials are necessary to validate this method as an alternative to the Draize rabbit eye irritation test method.