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α2,6-Sialyltransferase 과발현을 통한 인간형 시알산 부가 hCTLA4-Ig 생산 CHO 세포주 제작
임진혁(Jin-Hyuk Lim),차현명(Hyun-Myoung Cha),박혜진(Heajin Park),김하형(Ha Hyung Kim),김동일(Dong-Il Kim) 한국생물공학회 2017 KSBB Journal Vol.32 No.3
Sialylation is important in producing therapeutic proteins such as antibody, cytokine and fusion protein. Thus, enhancement of sialylation is usually performed in CHO cell cultures. α2,6-Sialyltransferase (ST), which plays a key role in the attachment of α2,6-sialic acid, is present in human cells but not in Chinese hamster ovary (CHO) cells. Overexpression of α2,6-ST can be used for enhancing the degree of sialylation and achieving human-like glycosylation. In this study, we constructed CHO cells producing human cytotoxic T-lymphocyte antigen4-immunoglobulin (hCTLA4-Ig) as well as α2,6- ST. Transfected CHO cells were selected using G418 and stable cell line was established. Profiles of viable cell density and hCTLA4-Ig titer in an overexpressed cell line were similar to those of a wild-type cell line. It was confirmed that the total amount of sialic acid was increased and α2,6-sialic acid was attached to the terminal residues of N-glycan of hCTLA4-Ig by ESI-LC-MS. Compared to 100% of α2,3-sialic acid in wild type cells, 70.9% of total sialylated N-glycans were composed of α2,6-sialic acid in transfected cells. In conclusion, overexpression of α2,6-ST in CHO cells led to the increase of both the amount of total sialylated N-glycan and the content of α2,6- sialic acid, which is more resemble to human-like structure of glycosylation.
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