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이태형 ( Tae Hyung Lee ),이은숙 ( Eunsuk Lee ),박태규 ( Taekyu Park ),홍성경 ( Seong Kyeong Hong ),김준호 ( Joon Ho Kim ) 한국안전학회(구 한국산업안전학회) 2014 한국안전학회지 Vol.29 No.4
It is crucial for important facilities to withstand strong earthquakes because their damage may cause undesirablesocio-economic effect. A liquefied natural gas (LNG) receiving terminal is one of the lifeline facilities whose seismic safety needs to beguaranteed. Even though all operating LNG receiving terminals in Korea were seismically designed, old design codes do not guarantee tocomply with the current seismic design codes. In addition, if the constructional materials have been deteriorated, the seismic capacity offacilities may be also deteriorated. Therefore, it is necessary that the seismic performance of LNG receiving terminals is evaluated and thefacilities that lack of seismic capacity have to be rehabilitated. In this paper, a procedure of seismic performance evaluation of suchfacilities is developed such that the procedure consists of three phases, namely pre-analysis, analysis, and evaluation phases. In thepre-analysis phase, design documents are reviewed and walk-on inspection is performed to determine the current state of the materialproperties. In the analysis phase, a structural analysis under a given earthquake or a seismic effect is performed to determine the seismicresponse of the structure. In the evaluation phase, seismic performance of the structure is evaluated based on limit states. Two of theimportant facilities, i.e. the submerged combustion vaporizer (SMV) and pipe racks of one of the Korean LNG receiving terminals areselected and evaluated according to the developed procedure. Both of the facilities are safe under the design level earthquake.
Cloning of Encoding Proteins that interact with the NK-1 Homeodomain Protein
Park,Taekyu,Lee,Kwang Ho 건국대학교 생명과학연구원 1995 생명과학지 Vol.2 No.-
초파리 NK-1 호미오박스(homeobox) 유전자는 특이 근육세포와 배부위 신경세포와 뇌에서 발현되는 것으로 알려졌다. NK-1 단백질과 상호작용하는 단백질을 발현하는 유전자를 알아내기 위하여 yeast two hybrid 시스템을 이용하여 초파리 match-maker cDNA library를 스크린하였다. GAL4 DNA 결합 부위에 NK-1이 들어 있는 pGBT9을 bait로 이용하였다. 히스티딘 결핍 배지에서 자라는 클론을 선택하여 베타 갈락토시다제 활성을 측정하여 단백질과 단백질의 상호작용을 확인하였다. 이 스크린에서 우리는 8개의 cDNA 클론을 얻었다. The Drosophila NK-1 homeobox gene was shown to be expressed in specific muscle cells and a subset of neuronal cells of the ventral nerve cord and brain. In order to identify genes which encode proteins that could interact with the NK-1 protein, we have screened a Drosophila math-maker cDNA library using the yeast two-hybrid system. Initially, pGBT9, which has the entire coding region of NK-1 fused to the GAL4 DNA-binding domain, was used as a bait. Clonies that can grow on Histidine-deficient media was selected, and β-galactosidase activity from these clonies were measured as an indication of protein-protein interaction. From this screening, we obtained eight cDNA clones.