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박종우,박윤미 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1
Platelet aggregation test was done by the impedance method and the optical method using Whole Blood Lumi-Aggregometer(Chrono-Log Corp., Havertown, PA). This study was performed to determine the optimal concentration of each aggregationing agent(ADP, collagen, ristocetin), maximal aggregability and aggregation time in each method, and evaluate the influence of other blood components in the impedance method. Method : Platelet aggregation test was performed by the impedence and optical methods in citrate whole blood from 20 normal volunteers Results : By examination, the following results were obtained 1. The maximal aggregabilities at the optimal reagent concentrations were 8.36±2.87ohm at ADP 12uM, 14.3±2.49ohm at collagen 5ug/mL, and 15.2±2.8ohm at ristocetin 1.25mg/mL in the impedance method, and 74±11% at ADP 6uM, 84.3±9.12% at collagen 5ug/mL, and 93.4±9.12% at ristocetin 1.25mg/mL in the optical method. 2. In normal controls the platelet aggregabilities were not affected by other blood components such as Hct, WBC and Platelet. 3. The average of the maximum aggregation time was 3.0-5.4 minutes by impedance method and 3.6-4.6 minutes in optical method in normal controls. Conclusion : In the platelet aggregation test, the impedance method is considered to be very useful to find out abnormal platelet function.