우 동결수정란의 산업적 이용에 관한 연구

노환철(H . C . Rho),정광업(K . E . Chung),신규용(G . Y . Shin),정병현(B . H . Chung),백운화(U . H . Pek),정길생(K . S . Chung) 한국축산학회 1988 한국축산학회지 Vol.30 No.3

To establish the industrial utilization of frozen bovine embryo, bovine embryos were frozen to -196℃, storaged, thawed and transferred. The results obtained from these experiments are summarized as follows : 1. Superovulation was induced from 27 Holstein donor cows. The average numbers of corpus luteum per donor cow was 10.3. The egg recovery rate was 56.3%, and the average number of recovered embryo was 4.6 per donor cow, 2. The pregnancy rates of frozen embryos by embryonic stages such as late morula, early blastocyst and blastocyst were 37.5%, 50.0%o and 42.9%, respectively, and those of fresh embryos were 55.0℃, 64.3% and 69.2%, respectively. 3. When fresh and frozen embryos were transferred to recipients whose estrus were naturally induced, the pregnancy rates were 53.3% and 36.8%, respectively. However, the pregnancy rates were 65.7% and 66.0%, respectively when fresh and frozen embryos were transferred to recipients whose estrus were artificially induced by PGF₂α. 4. When fresh and frozen embryos were transferred into tip of uterine horn, the pregnancy rates were 77.4% and 72.4%, respectively. On the other hand, pregnancy rates were 31.3% and 21.4%, respectively when fresh and frozen embryos were transferred into the bottom of uterine horn.

Anti-Inhibin Serum 및 Bovine Follicular Fluld 처리가 한우 난소의 난포 발달과 혈중 호르몬 변화에 미치는 영향

성환후,양병철,황성수,박성재,고응규,노환국,양보석,Seong, H.H.,Yang, B.C.,Hwang, S.S.,Park, S.J.,Ko, Y.G.,Rho, W.K.,Yang, B.S. 한국수정란이식학회 2006 한국동물생명공학회지 Vol.21 No.4

본 연구는 한우 난소의 난포 발달에 있어서 bFF 또는 anti-inhibin serum(AI)의 생리적 역할을 검토하기 위해 수행하였다. Saline(0.95%, control), bFF 또는 AI를 경정맥 주사 처리한 9마리의 한우 암소에서 채혈하여 혈중 estradiol-17$\beta$(E2), inhibin 및 progesterone(P4) 농도를 분석하였으며, 이들 처리에 의한 난포의 발달은 초음파 진단기를 이용하여 관찰하였다. 본 연구에서 얻어진 결과는 다음과 같다. 혈중 inhibin 농도는 bFF 처리구에서 배란 후 102시 간부터 유의적으로 증가하여(p<0.05) 지속적으로 높은 수준을 유지하였다. 혈중 E2 농도는 saline 처리구의 경우 bFF 처리구와 비슷한 수준으로 배란 후부터 낮은 농도를 유지하였으나, AI 처리구는 배란 후 36시간 이후부터 108시간까지 유의적으로 높은 수준을 유지하다가(p<0.05) 점차적으로 감소되는 경향을 나타내었다. 혈중 P4 농도는 saline 처리구와 AI 처리구에서 배란 후 68시간째부터 증가하기 시작하였으나, bFF 처리구에서는 배란 후 84시간부터 180시간까지 saline 또는 AI 처리구에 비해 유의적으로 낮은 농도를 나타내었다(p<0.05). 한편, AI 또는 bFF 처리가 난포 발달에 미치는 영향을 알아보기 위하여, 초음파 진단기를 이용하여 난소의 난포 발달을 조사한 결과, bFF 처리구에 비해 AI 처리구에서 발달 난포가 많은 것을 확인할 수 있었다. 이상의 결과를 종합해 보면, 외인성 AI는 한우난소에서 분비하는 inhibin을 특이하게 억제하여, inhibin에 의해 분비가 억제되어 있던 FSH 분비를 촉진시킴으로써 난포 발달과 estrogen의 분비 증가를 유발하는 것으로 사료된다 따라서 한우에 있어서 배란 직후 새롭게 발달하는 난포에는 적어도 inhibin이 관여하고 있는 것으로 사료된다. This study was performed to investigate the physiological effects of bovine follicular fluid (bFF) or anti-inhibin serum (AI) on follicular development in Hanwoo. Saline (0.95%), bFF or AI (total of 40 ml) were administered into the jugular vein in 9 Hanwoo cows. The plasma inhibin, estradiol-17 $\beta$ (E2), and progesterone (P4) levels were measured using RIA or ELISA kit and the number of ovarian follicles was observed by ultrasonography at 72 hr after ovulation. The plasma inhibin level in bFF treatment group was significantly increased and maintained higher level from 102 hr after ovulation compared to that of saline and AI groups (p<0.05). In plasma E2 level, AI treatment group showed significantly higher level from 36 hr to 108 hr after ovulation than that of saline and bFF groups (p<0.05). After that it showed decreasing tendency. The plasma P4 level was increased in control and AI treatment groups at 68 hr after ovulation. However, it was maintained significantly lower level in bFF group from 84 hr to 180 hr compared to that of saline and AI group (p<0.05). As a result of ultrasonography at 72 hr after ovulation, higher number of follicles was shown in AI treatment group compared to bFF groups, although the difference was not statistically significant. Taken together, it can be postulated that a treatment of synthesized AI inhibits the secretion of inhibin, stimulates FSH secretion inhibited by inhibin, and induces follicular development and estrogen secretion. According to these results, a development of ovarian follicle immediately after ovulation is associated closely with inhibin in Hanwoo heifers.

수정란이식에 의한 우 ( 牛 ) 의 쌍태유기에 관한 연구 4 . 회수된 수정란의 형태학적 고찰

정길생,정병현,노환철,윤종삼,정태영 ( Kil S . Chung,Byung H . Chung,Hwan C . Rho,Jong S . Yoon,Tae Y . Chung ) 한국축산학회 1983 한국축산학회지 Vol.25 No.5

Development stages and morphological characteristics of total of 179 bovine ova, non-surgically collected 8 to 9 days after estrus from 39 donor cattle superovulated with PMSG or FSH and Prostaglandin F₂α, were investigated under the phasecontrast microscope, and the following results were obtained. 1. Of 179 ova non-surgically collected from superovulated 39 donor cattle, 24(13.4%) were unfertilized. 2. Development stages of embryos at collection were ranged from morula (30.7%) to blastocyst (49.2%) or hatched blastocyst (6.7%). 3. In the morphological grading of 155 fertilized embryos, excellent, good, fair and poor embryos were 42(27.1%), 44(28.4%), 59(38.1%) and 10(6.4%), respectively. 4. The results described above suggest that the dosage of PMSG (3000IU) and the time of collecting embryo (8 to 9 days after estrus) adapted in this experiment were too much and late to get high percentage of morphologically excellent embryo.

수정란이식에 의한 우 ( 牛 )의 쌍태유기에 관한 연구 2 . 다배란처리의 반복이 난소반응과 수정란의 발달에 미치는 영향

정길생,박흠대,노환철,Richard A . Carmichael ( K . S . Chung,H . D . Park,H . C . Rho,Richard A . Carmichael ) 한국축산학회 1983 한국축산학회지 Vol.25 No.4

These experiments were carried out to clarify the effect of repeated superovulation in cattle on ovarian response and in vivo development of fertilized ova. Each cattle was repeatedly superovulated five times with the mixture of FSH and LH, and the growth of follicle, ovulation rate, the recovery rate of ovulated ova and in vivo development of fertilized ova following repeated superovulation were investigated. The results obtained were summarized as follows: 1. The numbers of mature follicles per cow following 1st and 5th superovulation were 20.7±8.60 and 13.6±3.64, respectively, showing the significant decrease (P$lt;0.05) in number of follicle growth with repeated hormone treatment for superovulation. 2. The percentage of ruptured follicles to the mature follicles was ranged 80.0 to 95.2%. No significant differences due to the treatment order were observed. 3. The numbers of ova recovered by non-surgical technique was ranged 60.8 to 64.5% to the number of functional corpus luteum and no significant differences were observed among treatments. The same was true for the percentage of normal embryo, which was ranged 69.5 to 72.8%.

생쥐 수정란의 양분에 의한 일란성 쌍태의 생산

김남형,정길생,노환철,백운화,이경광 ( N . H . Kim,K . S . Chung,H . C . Rho,U . H . Pek,K . K . Lee ) 한국축산학회 1986 한국축산학회지 Vol.28 No.8

These experiments were carried out to produce monozygotic twin by bisection of mouse morula. The morula was bisected by microglass needle without any aid of microinstrument or by lateral incision using sharp blade attached to micromanipulator. Bisected dermi-embryos were cultured and transferred to pseudopregnant recipient mouse. The results obtained in these experiments were summarized as follows: 1. Of 285 decompacted morulae bisected by microglass needle, 180 embryos were divided into two demi-embryos, respectively, without any visible damage. 2. Total 180 pairs of demi-embryos separated from decompacted morula and subjected to in vitro culture were resulted in 85(47.2%) pairs of eu-blastocyst, 40(22.2%) pairs of eu-blastocyst and pseudo-blastocyst, 35(19.4%) pairs of pseudo-blastocyst and 20(11.1%) pairs of trophectodermal vesicle and degeneration. 3. Of 255 intact morulae bisected by microblade, 100(39.2%) embryos were divided into two demi-embryos, respectively, without damage. However, 95(37.3%) embryos were bisected into one normal and one damaged demi-embryos, respectively. The percentage of demi-embryos developed to blastocyst after in vitro culture with and without zona pellucida were 71.4 and 64.9% respectively. 4. Total 15 twins were produced following transfer of the 38 pairs of eu-blastocysts developed from demi-embryos to 25 recipients. However, none of pseudo-blastocyst gave birth to young, 5. The percentage of eu-blastocyst cultured with or without zona pellucida and developed to live young following transfer were 11.1 and 9.3% respectively.