Benzo(a)pyrene과 Doxorubicin을 투여한 마우스 배아 섬유아 세포의 고농도 염으로 추출한 염색질 분획에서 DNA topoisomerase Ⅱ의 위치

이도근(DG Lee),김중근(JG Kim),정인철(IC Jung),조무연(MY Cho) 대한산부인과학회 1998 Obstetrics & Gynecology Science Vol.41 No.5

This study was conducted to determine if there exists a DNA topoisomerase II participating in the cleave and rejoining of DNA in the nuclear fraction of a mouse embryo fibroblast, C3H/10T1/2 cells to measure the expression of c-myc gene in the cells cultivated with benzo(a)pyrene (BP) and doxorubicin (Dx), together or alone, by means of Northern blot hybridization, and to observe changes of DNA topoisomerase II activity in chromatin subfraction extracted with high salt solution, and to confirm the existence of DNA topoisomerase II by Western blot immunodetection using the DNA topoisomerase II-antibody from the rabbit. The results are summarized as follows: 1. There was a region in the nuclear fraction of the C3H/10T1/2 cell showing a strong DNA topoisomerase II activity, which was included in the fraction insoluble to high salt (2M NaCl) solution. 2. In in vitro experiments, the relaxation activity of DNA topoisomerase II in pBR 322 DNA was increased by treatment with different concentration (1, 4, 10μM) of BP similar results were obserbed with BP/Dx treatment, although there was some mobility shift. 3. In Northern blot analysis, c-myc gene expression was increased in C3H/10T1/2 cell treated BP or Dx, but it decreased in cells treated with BP and Dx together. 4. Centrifugation of 2M NaCl chromatin extraction of C3H/10T1/2 cell treated with BP, Dx or BP/Dx yielded two components, DNA-S and DNA-P. In case of BP treated, the topoisomerase activity was exist in both fraction but the group with Dx was activity in DNA-S only when the enzyme activity was determined in the presence of Dx, it was only higher than that of BP in DNA-S fraction. 5. The Western blot immunodetection of the above fractions indicated that DNA topoisomerase II existed in DNA-S and DNA-P fractions for the BP treated group but Dx trated group was in DNA-S fraction only. However, the amount of enzyme in the DNA-S of BP/Dx treated group were increased to compare with Dx treated group. These results indicated that the high salt insoluble fraction was representative region to topoisomerase II activity, also the DNA-S fraction by the action of Dx may play an important role in the function of a gene.

총배설강 외번증 1 례

권해석(HS Kuen),박성춘(SC Park),김중근(JG Kim),곽태로(TR Gwag),백옥기(OK Paik) 대한산부인과학회 1985 Obstetrics & Gynecology Science Vol.28 No.6

Cloacal exstrophy is a rare congenital marformation and a distinctive morphologic syndrome. The cardinal clinical features of cloacal exstrohy consist of exstrophy of the bladder, omphalocele, prolapse of the intestine, imperforate anus and lumbosacral myelocele. The essential feature of the syndrome is a failure of cloacal separation at 4~5 weeks of gestation, which results in a persistent cloaca into which the ureter and a rudimentary hindgut open. Most cases of cloacal exstrophy are sporadic, and none has been shown to be genetic in origin. Surgical treatment has been carried with limited effectiveness. The prognosis may be hopeless in a significant number of these infant. Recently we have experienced a case of exstrophy of cloaca in Korea for the first time and we are to report it with brief review of literature.

선택적 제왕절개 분만시 전신마취와 경막외 마취의 임상통계학적 고찰

이명선(MS Lee),김정대(JD Kim),이도근(DG Lee),윤병철(BC Yoon),김중근(JG Kim) 대한산부인과학회 1998 Obstetrics & Gynecology Science Vol.41 No.2

This study was undertaken to evaluate the effects of general and epidural anesthesia for cesarean section. Controversy exsists concerning the choice anesthetic technic for elective cesarean section. Several maternal and newborn parameters were compared during general anesthesia and epidural anesthesia. One hundred thirteen paturients for elective cesarean section was divided into two groups. One group(56) of paturients was anesthetized with general anesthesia. The other group(57) was anesthetized with epidural anesthesia. The results were as follows; 1) There was no significant difference in age, weight, height, blood pressure, pulse rate, hemoglobin and gravidity of paturients, weight of newborn except gestational age of paturients between two groups before cesarean section. 2) There was no significant difference in total surgical time between two groups but induction of anesthesia-delivery time is prolonged in epidural anesthesia. 3) For elective cesarean section under general anesthesia, prolongation of induction-delivery time was not related to lowering of Apgar scores. 4) For elective cesarean section under epidural anesthesia, there was no more neonatal depression accompanied by prolongation of local analgesic injection-delivery time. 5) There was no significant difference in one minute Apgar score and five minute Apgar score between two groups. 6) Maternal PaO2 at the time of uterine incision was significantly higher in the epidural group. But the pH of both umbilical artery and vein was significantly lower in the epidural group. There was a greater(but not significantly greater)base deficit in the umbilical-cord samples of the general anesthesia group. Transplacental difference were statistically significant for O2 alone. 7) In contrast to epidural group, general anesthesia patients developed an impressive degree of hypertension. But hypotension did not develop during epidural anesthesia. 8) There was no significant difference in mean blood loss during operation and lowering of hemoglobin after 24 hours between two groups. 9) Urinary retention, which occured in 26.3% of patients during epidural anesthesia, is only statistically significant difference complication. 10) Forty patient experienced no pain or discomfort during epidural anesthesia. On the basis of this study, neither technique can be vigorously recommended over the other from the standpoint of the newborn condition and maternal status. But we wish to evaluate the study of epidural anesthesia for cesarean section.

자궁경관임신의 1 례

유금준(GJ Yoo),강경주(KJ Kang),김중근(JG Kim),곽태로(TR Gwag) 대한산부인과학회 1987 Obstetrics & Gynecology Science Vol.30 No.6

저자들은 개인병원에서 소파수술후 지속적인 성기출혈을 주소로하여 내원한 47세의 경산부에서 희귀한 자궁경관임신을 경험하여 자궁전적출술을 시행하였고 병리조직학적 검사에서 확진되었기에 문헌적 고찰과 함께 보고하는 바이다 A case of cervial pregnancy which is recently experienced in our department is presented with a brief review of related literatures

난소낭성기형종의 임상 및 병리조직학적 고찰

손성락(SR Son),전지혜(JH Jeon),문태식(TS Moon),김중근(JG Kim) 대한산부인과학회 1994 Obstetrics & Gynecology Science Vol.37 No.10

A clinical and histopathological study was made on a series of 63 ovarian cystic teratomas, operatied upon and confirmed by histopathological examination during 5 years from Jan. 1989 till Dec. 1993. The results were as follows: 1. The frequency of ovarian cystic teratoma was 27.8% of all ovarian tumors, operated. 2. The incidence of ovarian cystic teratoma was greatest in third decade(44.5%). 3. The bilaterality was 17.5%, right site 46.0%, left sited 36.5%. 4. The average diameter was 7 cm, about 3/4 (75.7%) was 5-9cm. 5. The most common symptoms were abdominal pain or discomfort(50.8%) and palpable mass (30.2%), and no symptom in 20.6%. 6. Pregnancy was associated in 12.7%, and the rest were PID and uterine myoma associated. 7. Complications were torsion(12.7%), rupture(3.1%), infection(3.1%). 8. The structures observed microscopically were skin(100%), and skin appendages(97.39%), largely institued.

45 , XO/46 , XX/47 , XXX Mosaicism을 보이는 월발성무월경 1 례

전지혜(JH Jeon),이명선(MS Lee),김정대(JD Kim),이도근(DG Lee),지홍련(HL Jee),김중근(JG Kim) 대한산부인과학회 1997 Obstetrics & Gynecology Science Vol.40 No.2

There are many variant forms in Turner`s syndrome which is characterized by female phenotype, short height, primary amenorrhea, infertility, abnormal secondary sexual development, and mental retardation. Among these 45,XO/46,XX/47,XXX form is known as rare variant of Turner`s syndrome. 45,XO/46,XX/47,XXX mosaicism is characterized by normal height, possibility of normal sexual development, menstrual/pregnancy history and normal intelligence. We report a cse of 45,XO/46,XX/47,XXX Turner`s syndrome associated with primary amenorrhea in 17-year old Korean female.