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Strobilurin계 살균제에 대한 잿빛곰팡이병균 Botrytis cinerea의 저항성 검정
김아형(Ah Hyeong Kim),김선보(Seon Bo Kim),한기돈(Ki Don Han),김흥태(Heung Tae Kim) 한국농약과학회 2014 농약과학회지 Vol.18 No.3
This study was conducted to investigate the resistance of Botrytis cinerea to azoxystrobin, which belongs to strobilurin fungicides. The sensitivity of B. cinerea isolates, which were collected from infected pepper, strawberry, cucumber and tomato by a single spore isolation, to the fungicide was tested through a agar dilution method on PDA amended with fungicides and 100 μg mL<SUP>?1</SUP> of salicylhydroxamic acid (SHAM). All isolates of B. cinerea tested in this study were classified as a sensitive and a resistant group by 1.0 μg mL<SUP>?1</SUP> of EC50 value to azoxystrobin. While the sensitive isolates accounted for 46.5% of B. cinerea population, the resistant ones did for 53.5%. According to the regions isolating B. cinerea, the highest isolation frequency was showed as 81.1% in Chungnam among the all. Among 4 host plants as pepper, strawberry, cucumber and tomato, the highest isolation frequency was obtained in strawberry, while the lowest was done in pepper. The isolate resistant to azoxystrobin showed the cross resistance to other fungicides included into strobilurins as kresoxim-methyl and trifloxystrobin. In spite of an excellent efficacy of strobilurins, it should be taken care to use them in the field, because of the high risk in the fields.
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Mepanipyrim에 대한 잿빛곰팡이병균의 저항성 검정
김아형(Ah Hyeong Kim),김선보(Seon Bo Kim),한기돈(Kee Don Han),김흥태(Heung Tae Kim) 한국농약과학회 2015 농약과학회지 Vol.19 No.3
With 86 isolates of Botrytis cinerea obtained from diseased fruits of tomato, strawberry and cucumber, it was conducted to detect the fungicide resistance of B. cinerea against mepanipyrim through an agar dilution method. FGA medium was used for monitoring the resistance. Among 86 isolates of B. cinerea, resistant isolates were composed by 23.3%, which was different according to regions isolating the pathogen. In accordance with isolation region, the isolation frequency of resistant isolates was as follows; that of Gyeonggi, Gyeongnam/Pusan and chungnam was 28.6%, 33.3% and 12.8%, respectively. The resistant isolates of B. cinerea showed the cross resistance between mepanipyrim and pyrimethanil. Also the pathogenicity of B. cinerea resistant to mepanipyrim was high as like that of sensitive isolates. Because of them, it should be necessary to manage the spraying system of mepanipyrim and the monitoring for the fungicide resistance.
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